Detailed information of TA system TA055390

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 1677181..1677612
• Replicon: chromosome
• Accession: NZ_CP011994
• Organism: Ectothiorhodospira sp. BSL-9

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: ECTOBSL9_RS16545
• Protein ID: WP_205632006.1
• Coordinates: 1677181..1677351 (+)
• Length: 57 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A0A161HFY6
• Locus tag: ECTOBSL9_RS07845
• Protein ID: WP_063464598.1
• Coordinates: 1677358..1677612 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECTOBSL9_RS07820	1672270..1673337	+	1068	WP_063464593.1	DDE-type integrase/transposase/recombinase	-
ECTOBSL9_RS07825	1673342..1674142	+	801	WP_063464594.1	AAA family ATPase	-
ECTOBSL9_RS07830	1674139..1674345	+	207	WP_063464595.1	hypothetical protein	-
ECTOBSL9_RS07835	1674766..1675509	+	744	WP_063464596.1	PEP-CTERM sorting domain-containing protein	-
ECTOBSL9_RS07840	1675626..1676996	-	1371	WP_063464597.1	HipA domain-containing protein	-
ECTOBSL9_RS16545	1677181..1677351	+	171	WP_205632006.1	BrnT family toxin	Toxin
ECTOBSL9_RS07845	1677358..1677612	+	255	WP_063464598.1	BrnA antitoxin family protein	Antitoxin
ECTOBSL9_RS16550	1677764..1678076	-	313	Protein_1578	transposase	-
ECTOBSL9_RS07850	1678115..1679515	+	1401	WP_063464599.1	transposase	-
ECTOBSL9_RS07855	1679528..1679707	+	180	WP_063464600.1	hypothetical protein	-
ECTOBSL9_RS07860	1679822..1681387	+	1566	WP_063464601.1	ATP-binding protein	-
ECTOBSL9_RS17305	1681397..1681564	-	168	WP_205632031.1	transcriptional regulator	-
ECTOBSL9_RS07870	1681822..1682136	-	315	WP_063464603.1	type II toxin-antitoxin system HigB family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1637224..1694716	57492

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-54)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (24-77)

Sequences

Toxin

Protein

• Length: 57 a.a.
• Molecular weight: 6837.76 Da
• Isoelectric Point: 6.8679

>T55390 WP_205632006.1 NZ_CP011994:1677181-1677351 [Ectothiorhodospira sp. BSL-9]
MRFEWDEAKNQANIRKHGIDFNDVPDIFQHPMLALHDGRMHYGEERWICIGWISRR

Nucleotide

• Length: 171 bp

>T55390 NZ_CP011994:1677181-1677351 [Ectothiorhodospira sp. BSL-9]
ATGCGGTTTGAGTGGGACGAAGCCAAGAATCAGGCCAACATTCGCAAGCATGGTATCGACTTCAATGACGTGCCGGACAT
CTTCCAGCATCCGATGCTGGCCTTGCATGATGGCCGAATGCACTACGGCGAAGAGCGTTGGATCTGCATCGGCTGGATCA
GCAGGAGGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9991.33 Da
• Isoelectric Point: 8.4931

>AT55390 WP_063464598.1 NZ_CP011994:1677358-1677612 [Ectothiorhodospira sp. BSL-9]
MSKASKTDWERLAKMNDEDIDTQDMPELGEDFFRRAELRVPVKKAVTIRLDADVLEWFKGQGAGYQTRINQLLRHYMNAQ
QRYR

Nucleotide

• Length: 255 bp

>AT55390 NZ_CP011994:1677358-1677612 [Ectothiorhodospira sp. BSL-9]
ATGTCGAAAGCATCGAAAACTGATTGGGAACGGCTGGCCAAGATGAACGACGAGGACATCGATACCCAAGACATGCCTGA
ATTGGGCGAAGACTTCTTTCGGCGCGCCGAGCTACGTGTGCCGGTAAAGAAGGCGGTGACAATCCGTCTCGACGCCGATG
TGCTGGAGTGGTTCAAGGGCCAGGGCGCGGGCTACCAGACTCGCATCAATCAGTTGTTGCGCCATTACATGAATGCCCAG
CAGCGGTATCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A161HFY6