Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 16817..17553 Replicon plasmid pUHKPC07-74.026kb
Accession NZ_CP011987
Organism Klebsiella pneumoniae UHKPC07

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag H224_RS27630 Protein ID WP_003026803.1
Coordinates 17071..17553 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag H224_RS27625 Protein ID WP_003026799.1
Coordinates 16817..17083 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H224_RS27580 12285..13028 - 744 WP_000129823.1 hypothetical protein -
H224_RS27585 13079..13429 - 351 WP_000493378.1 hypothetical protein -
H224_RS27590 13999..14268 + 270 WP_004152102.1 hypothetical protein -
H224_RS27595 14256..14831 + 576 WP_004152103.1 hypothetical protein -
H224_RS27600 14862..15356 + 495 WP_004152104.1 DNA-binding protein -
H224_RS27605 15400..15768 + 369 WP_004152105.1 hypothetical protein -
H224_RS27610 15802..16005 + 204 WP_004152106.1 hemolysin expression modulator Hha -
H224_RS27615 16054..16311 + 258 WP_004152107.1 hypothetical protein -
H224_RS27620 16387..16641 + 255 WP_004152108.1 hypothetical protein -
H224_RS27625 16817..17083 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
H224_RS27630 17071..17553 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
H224_RS27635 17765..19111 + 1347 WP_020314316.1 ISNCY family transposase -
H224_RS30125 19612..20866 - 1255 Protein_27 IS3 family transposase -
H224_RS27650 20954..21916 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-Ib / blaSHV-12 - 1..74026 74026


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T55384 WP_003026803.1 NZ_CP011987:17071-17553 [Klebsiella pneumoniae UHKPC07]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T55384 NZ_CP011987:17071-17553 [Klebsiella pneumoniae UHKPC07]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT55384 WP_003026799.1 NZ_CP011987:16817-17083 [Klebsiella pneumoniae UHKPC07]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT55384 NZ_CP011987:16817-17083 [Klebsiella pneumoniae UHKPC07]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References