Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 32173..32816 Replicon plasmid pUHKPC07-113.639kb
Accession NZ_CP011986
Organism Klebsiella pneumoniae UHKPC07

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag H224_RS27015 Protein ID WP_001044770.1
Coordinates 32173..32589 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag H224_RS27020 Protein ID WP_001261282.1
Coordinates 32586..32816 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H224_RS26975 27715..27951 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
H224_RS26980 27948..28310 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
H224_RS26985 28328..30022 - 1695 WP_000105636.1 mercury(II) reductase -
H224_RS26990 30074..30496 - 423 WP_001340589.1 organomercurial transporter MerC -
H224_RS26995 30532..30657 - 126 WP_072093211.1 mercury transporter -
H224_RS27000 30658..31355 - 698 Protein_32 IS1 family transposase -
H224_RS27010 31389..32099 - 711 WP_004152334.1 ATP-binding protein -
H224_RS27015 32173..32589 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
H224_RS27020 32586..32816 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H224_RS27025 32773..33234 + 462 WP_072093212.1 hypothetical protein -
H224_RS27030 33469..33675 + 207 WP_004153649.1 hypothetical protein -
H224_RS27035 33721..34029 + 309 WP_004152336.1 hypothetical protein -
H224_RS27040 34057..34386 + 330 WP_004152337.1 hypothetical protein -
H224_RS27045 34412..34810 + 399 WP_004171440.1 hypothetical protein -
H224_RS27050 34817..35149 + 333 WP_004152339.1 hypothetical protein -
H224_RS27055 35149..35931 + 783 WP_004152340.1 site-specific integrase -
H224_RS29235 37145..37618 - 474 WP_004152341.1 YkgJ family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaOXA-9 / blaTEM-1A - 1..113639 113639
- flank IS/Tn - - 37738..39057 1319


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T55383 WP_001044770.1 NZ_CP011986:c32589-32173 [Klebsiella pneumoniae UHKPC07]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T55383 NZ_CP011986:c32589-32173 [Klebsiella pneumoniae UHKPC07]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT55383 WP_001261282.1 NZ_CP011986:c32816-32586 [Klebsiella pneumoniae UHKPC07]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT55383 NZ_CP011986:c32816-32586 [Klebsiella pneumoniae UHKPC07]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References