Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4045544..4046163 Replicon chromosome
Accession NZ_CP011985
Organism Klebsiella pneumoniae UHKPC07

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag H224_RS20435 Protein ID WP_002892050.1
Coordinates 4045945..4046163 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag H224_RS20430 Protein ID WP_002892066.1
Coordinates 4045544..4045918 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H224_RS20420 4040696..4041889 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H224_RS20425 4041912..4045058 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
H224_RS20430 4045544..4045918 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
H224_RS20435 4045945..4046163 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
H224_RS20440 4046322..4046888 + 567 WP_002892030.1 maltose O-acetyltransferase -
H224_RS30600 4046860..4047000 - 141 WP_004147370.1 hypothetical protein -
H224_RS20450 4047021..4047491 + 471 WP_002892026.1 YlaC family protein -
H224_RS20455 4047466..4048917 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
H224_RS20460 4049018..4049716 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
H224_RS20465 4049713..4049853 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
H224_RS20470 4049853..4050116 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T55378 WP_002892050.1 NZ_CP011985:4045945-4046163 [Klebsiella pneumoniae UHKPC07]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T55378 NZ_CP011985:4045945-4046163 [Klebsiella pneumoniae UHKPC07]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT55378 WP_002892066.1 NZ_CP011985:4045544-4045918 [Klebsiella pneumoniae UHKPC07]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT55378 NZ_CP011985:4045544-4045918 [Klebsiella pneumoniae UHKPC07]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References