Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 41262..41998 Replicon plasmid p500_1420-130.552kb
Accession NZ_CP011981
Organism Klebsiella pneumoniae 500_1420

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag J052_RS26970 Protein ID WP_003026803.1
Coordinates 41262..41744 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag J052_RS26975 Protein ID WP_003026799.1
Coordinates 41732..41998 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J052_RS26950 36899..37861 + 963 WP_004152113.1 M48 family metalloprotease -
J052_RS29375 38002..38481 + 480 WP_071527923.1 transposase -
J052_RS26960 38478..39203 + 726 WP_004152111.1 IS3 family transposase -
J052_RS26965 39704..41050 - 1347 WP_020314316.1 ISNCY family transposase -
J052_RS26970 41262..41744 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
J052_RS26975 41732..41998 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
J052_RS26980 42174..42428 - 255 WP_004152108.1 hypothetical protein -
J052_RS26985 42504..42761 - 258 WP_004152107.1 hypothetical protein -
J052_RS26990 42810..43004 - 195 WP_052536218.1 hemolysin expression modulator Hha -
J052_RS26995 43118..44098 - 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
J052_RS27000 44247..44615 - 369 WP_004152105.1 hypothetical protein -
J052_RS27005 44659..45153 - 495 WP_004152104.1 DNA-binding protein -
J052_RS27010 45184..45759 - 576 WP_004152103.1 hypothetical protein -
J052_RS27015 45747..46016 - 270 WP_004152102.1 hypothetical protein -
J052_RS29380 46191..46442 + 252 Protein_52 hypothetical protein -
J052_RS27025 46677..46883 + 207 WP_004153649.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 - 1..130552 130552
- inside IScluster/Tn - - 38176..44098 5922


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T55364 WP_003026803.1 NZ_CP011981:c41744-41262 [Klebsiella pneumoniae 500_1420]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T55364 NZ_CP011981:c41744-41262 [Klebsiella pneumoniae 500_1420]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT55364 WP_003026799.1 NZ_CP011981:c41998-41732 [Klebsiella pneumoniae 500_1420]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT55364 NZ_CP011981:c41998-41732 [Klebsiella pneumoniae 500_1420]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References