Detailed information of TA system TA055363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5230306..5230937
• Replicon: chromosome
• Accession: NZ_CP011980
• Organism: Klebsiella pneumoniae 500_1420

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: J052_RS26375
• Protein ID: WP_002882817.1
• Coordinates: 5230306..5230689 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J052_RS26380
• Protein ID: WP_002882814.1
• Coordinates: 5230689..5230937 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J052_RS26360	5227672..5228574	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
J052_RS26365	5228571..5229206	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
J052_RS26370	5229203..5230132	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
J052_RS26375	5230306..5230689	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
J052_RS26380	5230689..5230937	-	249	WP_002882814.1	ribbon-helix-helix domain-containing protein	Antitoxin
J052_RS26385	5231136..5232053	+	918	WP_002882812.1	alpha/beta hydrolase	-
J052_RS26390	5232067..5233008	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
J052_RS26395	5233053..5233490	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
J052_RS26400	5233487..5234347	-	861	WP_002882807.1	virulence factor BrkB family protein	-
J052_RS26405	5234341..5234940	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (18-49)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T55363 WP_002882817.1 NZ_CP011980:c5230689-5230306 [Klebsiella pneumoniae 500_1420]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T55363 NZ_CP011980:c5230689-5230306 [Klebsiella pneumoniae 500_1420]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9786.10 Da
• Isoelectric Point: 4.3509

>AT55363 WP_002882814.1 NZ_CP011980:c5230937-5230689 [Klebsiella pneumoniae 500_1420]
MSMMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRK
EW

Nucleotide

• Length: 249 bp

>AT55363 NZ_CP011980:c5230937-5230689 [Klebsiella pneumoniae 500_1420]
ATGAGCATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCT
CAAAGTGCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTA
AAGATACCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAG
GAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8