Detailed information of TA system TA055345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 8873..9362
• Replicon: plasmid pDMC1097-77.775kb
• Accession: NZ_CP011978
• Organism: Klebsiella pneumoniae DMC1097

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U3PL64
• Locus tag: H218_RS28355
• Protein ID: WP_020314676.1
• Coordinates: 8873..9133 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: H218_RS30325
• Protein ID: WP_022540736.1
• Coordinates: 9123..9362 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H218_RS30315	4961..5290	+	330	WP_001542022.1	hypothetical protein	-
H218_RS28320	5694..6209	+	516	WP_001025393.1	J domain-containing protein	-
H218_RS28325	6313..6855	+	543	WP_001083904.1	hypothetical protein	-
H218_RS28330	6852..7106	+	255	WP_001230709.1	hypothetical protein	-
H218_RS28335	7103..7345	+	243	WP_000151456.1	hypothetical protein	-
H218_RS28345	7926..8249	+	324	WP_020314677.1	hypothetical protein	-
H218_RS28350	8291..8500	+	210	WP_020314678.1	hypothetical protein	-
H218_RS30320	8597..8821	+	225	WP_000814381.1	hypothetical protein	-
H218_RS28355	8873..9133	+	261	WP_020314676.1	hypothetical protein	Toxin
H218_RS30325	9123..9362	+	240	WP_022540736.1	transcriptional regulator	Antitoxin
H218_RS28360	9897..10157	+	261	WP_020314977.1	hypothetical protein	-
H218_RS28365	10544..10726	-	183	WP_001401700.1	hypothetical protein	-
H218_RS30340	11271..11453	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
H218_RS30345	11478..11915	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
H218_RS28370	12046..12366	-	321	WP_001310435.1	hypothetical protein	-
H218_RS28375	12363..12878	-	516	WP_001229189.1	hypothetical protein	-
H218_RS28380	12915..13178	-	264	WP_020314988.1	hypothetical protein	-
H218_RS28385	13180..13599	-	420	WP_001708405.1	hypothetical protein	-
H218_RS28390	13619..14326	-	708	WP_000108796.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-3 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A	-	1..77775	77775

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9922.30 Da
• Isoelectric Point: 7.2028

>T55345 WP_020314676.1 NZ_CP011978:8873-9133 [Klebsiella pneumoniae DMC1097]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRDNDKEIVFMMIQEMS
EEENAN

Nucleotide

• Length: 261 bp

>T55345 NZ_CP011978:8873-9133 [Klebsiella pneumoniae DMC1097]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGATAATGATAAAGAAATTGTTTTTATGATGATTCAGGAAATGAGC
GAAGAAGAAAATGCTAACTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9072.61 Da
• Isoelectric Point: 10.2613

>AT55345 WP_022540736.1 NZ_CP011978:9123-9362 [Klebsiella pneumoniae DMC1097]
MLTENTPENIKRLRKKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKYLLLLAGEHPEYVLSKRDRGILK

Nucleotide

• Length: 240 bp

>AT55345 NZ_CP011978:9123-9362 [Klebsiella pneumoniae DMC1097]
ATGCTAACTGAAAACACACCAGAAAACATTAAGCGTCTGCGGAAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTCAGCATGAGTCCGCGTACATGGCGTCGTAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCCGTGG
AGTTTAAATATCTTCTGCTTTTAGCTGGGGAACATCCTGAATACGTCCTTAGTAAAAGAGATCGAGGAATTTTAAAATGA

Structures

Toxin

• AlphaFold DB: A0A376MG86