Detailed information of TA system TA055344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 6852..7345
• Replicon: plasmid pDMC1097-77.775kb
• Accession: NZ_CP011978
• Organism: Klebsiella pneumoniae DMC1097

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: H218_RS28330
• Protein ID: WP_001230709.1
• Coordinates: 6852..7106 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: H218_RS28335
• Protein ID: WP_000151456.1
• Coordinates: 7103..7345 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H218_RS30305	2426..2587	+	162	WP_001434039.1	type I toxin-antitoxin system Hok family toxin	-
H218_RS28305	2737..3333	+	597	WP_000125164.1	hypothetical protein	-
H218_RS32820	3351..3635	-	285	WP_001162371.1	hypothetical protein	-
H218_RS28315	3709..4374	-	666	WP_000532148.1	P-loop NTPase	-
H218_RS30315	4961..5290	+	330	WP_001542022.1	hypothetical protein	-
H218_RS28320	5694..6209	+	516	WP_001025393.1	J domain-containing protein	-
H218_RS28325	6313..6855	+	543	WP_001083904.1	hypothetical protein	-
H218_RS28330	6852..7106	+	255	WP_001230709.1	hypothetical protein	Toxin
H218_RS28335	7103..7345	+	243	WP_000151456.1	hypothetical protein	Antitoxin
H218_RS28345	7926..8249	+	324	WP_020314677.1	hypothetical protein	-
H218_RS28350	8291..8500	+	210	WP_020314678.1	hypothetical protein	-
H218_RS30320	8597..8821	+	225	WP_000814381.1	hypothetical protein	-
H218_RS28355	8873..9133	+	261	WP_020314676.1	hypothetical protein	-
H218_RS30325	9123..9362	+	240	WP_022540736.1	transcriptional regulator	-
H218_RS28360	9897..10157	+	261	WP_020314977.1	hypothetical protein	-
H218_RS28365	10544..10726	-	183	WP_001401700.1	hypothetical protein	-
H218_RS30340	11271..11453	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
H218_RS30345	11478..11915	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-3 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A	-	1..77775	77775

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T55344 WP_001230709.1 NZ_CP011978:6852-7106 [Klebsiella pneumoniae DMC1097]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T55344 NZ_CP011978:6852-7106 [Klebsiella pneumoniae DMC1097]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT55344 WP_000151456.1 NZ_CP011978:7103-7345 [Klebsiella pneumoniae DMC1097]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT55344 NZ_CP011978:7103-7345 [Klebsiella pneumoniae DMC1097]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5