Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 80639..81164 Replicon plasmid pDMC1097-218.836kb
Accession NZ_CP011977
Organism Klebsiella pneumoniae DMC1097

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag H218_RS27545 Protein ID WP_001568026.1
Coordinates 80859..81164 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag H218_RS27540 Protein ID WP_001568025.1
Coordinates 80639..80857 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H218_RS27500 75927..76184 - 258 WP_004152107.1 hypothetical protein -
H218_RS27505 76233..76436 - 204 WP_004152106.1 hemolysin expression modulator Hha -
H218_RS27510 76470..76838 - 369 WP_004152105.1 hypothetical protein -
H218_RS27515 76882..77376 - 495 WP_004152104.1 DNA-binding protein -
H218_RS27520 77407..77982 - 576 WP_004152103.1 hypothetical protein -
H218_RS27525 77970..78239 - 270 WP_004152102.1 hypothetical protein -
H218_RS27530 78676..79365 + 690 WP_020315560.1 RES family NAD+ phosphorylase -
H218_RS27535 79397..80086 - 690 WP_004193995.1 hypothetical protein -
H218_RS27540 80639..80857 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H218_RS27545 80859..81164 + 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
H218_RS27550 81333..81728 + 396 WP_017899885.1 hypothetical protein -
H218_RS27555 81755..82078 + 324 WP_022644730.1 hypothetical protein -
H218_RS27560 82075..83091 + 1017 WP_020315256.1 hypothetical protein -
H218_RS27565 83289..84083 + 795 WP_004197635.1 site-specific integrase -
H218_RS30720 84499..84678 - 180 WP_006788217.1 hypothetical protein -
H218_RS27570 84798..85424 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA12 / aadA2 / qacE / sul1 / mph(A) / aph(3')-Ia / blaSHV-12 - 1..218836 218836


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T55343 WP_001568026.1 NZ_CP011977:80859-81164 [Klebsiella pneumoniae DMC1097]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T55343 NZ_CP011977:80859-81164 [Klebsiella pneumoniae DMC1097]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT55343 WP_001568025.1 NZ_CP011977:80639-80857 [Klebsiella pneumoniae DMC1097]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT55343 NZ_CP011977:80639-80857 [Klebsiella pneumoniae DMC1097]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References