Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 74685..75421 Replicon plasmid pDMC1097-218.836kb
Accession NZ_CP011977
Organism Klebsiella pneumoniae DMC1097

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag H218_RS27485 Protein ID WP_003026803.1
Coordinates 74685..75167 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag H218_RS27490 Protein ID WP_003026799.1
Coordinates 75155..75421 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H218_RS27465 70322..71284 + 963 WP_004152113.1 M48 family metalloprotease -
H218_RS31280 71372..72626 + 1255 Protein_78 IS3 family transposase -
H218_RS27480 73127..74473 - 1347 WP_020314316.1 ISNCY family transposase -
H218_RS27485 74685..75167 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
H218_RS27490 75155..75421 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
H218_RS27495 75597..75851 - 255 WP_004152108.1 hypothetical protein -
H218_RS27500 75927..76184 - 258 WP_004152107.1 hypothetical protein -
H218_RS27505 76233..76436 - 204 WP_004152106.1 hemolysin expression modulator Hha -
H218_RS27510 76470..76838 - 369 WP_004152105.1 hypothetical protein -
H218_RS27515 76882..77376 - 495 WP_004152104.1 DNA-binding protein -
H218_RS27520 77407..77982 - 576 WP_004152103.1 hypothetical protein -
H218_RS27525 77970..78239 - 270 WP_004152102.1 hypothetical protein -
H218_RS27530 78676..79365 + 690 WP_020315560.1 RES family NAD+ phosphorylase -
H218_RS27535 79397..80086 - 690 WP_004193995.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA12 / aadA2 / qacE / sul1 / mph(A) / aph(3')-Ia / blaSHV-12 - 1..218836 218836


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T55342 WP_003026803.1 NZ_CP011977:c75167-74685 [Klebsiella pneumoniae DMC1097]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T55342 NZ_CP011977:c75167-74685 [Klebsiella pneumoniae DMC1097]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT55342 WP_003026799.1 NZ_CP011977:c75421-75155 [Klebsiella pneumoniae DMC1097]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT55342 NZ_CP011977:c75421-75155 [Klebsiella pneumoniae DMC1097]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References