Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 48415..49058 Replicon plasmid pDMC1097-218.836kb
Accession NZ_CP011977
Organism Klebsiella pneumoniae DMC1097

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag H218_RS27340 Protein ID WP_000754566.1
Coordinates 48642..49058 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag H218_RS27335 Protein ID WP_001261276.1
Coordinates 48415..48645 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H218_RS27300 43534..43803 + 270 WP_000339857.1 hypothetical protein -
H218_RS27305 43980..44846 - 867 WP_004118283.1 replication initiation protein -
H218_RS32550 45376..45480 + 105 WP_032409716.1 hypothetical protein -
H218_RS27315 45609..45866 + 258 WP_000764642.1 hypothetical protein -
H218_RS27320 45924..46700 - 777 WP_000015958.1 site-specific integrase -
H218_RS27325 46697..47440 - 744 WP_000129823.1 hypothetical protein -
H218_RS27330 47491..47841 - 351 WP_000493378.1 hypothetical protein -
H218_RS27335 48415..48645 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H218_RS27340 48642..49058 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
H218_RS30700 49262..50118 + 857 Protein_54 IS3-like element ISEc15 family transposase -
H218_RS27355 50400..50762 + 363 Protein_55 transposase -
H218_RS27360 50795..53800 - 3006 WP_001143775.1 Tn3-like element Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA12 / aadA2 / qacE / sul1 / mph(A) / aph(3')-Ia / blaSHV-12 - 1..218836 218836


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T55341 WP_000754566.1 NZ_CP011977:48642-49058 [Klebsiella pneumoniae DMC1097]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T55341 NZ_CP011977:48642-49058 [Klebsiella pneumoniae DMC1097]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT55341 WP_001261276.1 NZ_CP011977:48415-48645 [Klebsiella pneumoniae DMC1097]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT55341 NZ_CP011977:48415-48645 [Klebsiella pneumoniae DMC1097]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References