Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 275010..275647 Replicon chromosome
Accession NZ_CP011974
Organism Priestia filamentosa strain Hbe603

Toxin (Protein)


Gene name pemK Uniprot ID A0A329EQQ9
Locus tag BEH_RS01420 Protein ID WP_019395220.1
Coordinates 275297..275647 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A329EU69
Locus tag BEH_RS01415 Protein ID WP_019395219.1
Coordinates 275010..275291 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEH_RS01390 270160..271137 + 978 WP_046218255.1 UV DNA damage repair endonuclease UvsE -
BEH_RS01395 271160..271747 - 588 WP_046218254.1 rhomboid family intramembrane serine protease -
BEH_RS01400 271920..272276 + 357 WP_019395216.1 holo-ACP synthase -
BEH_RS01405 272378..273385 + 1008 WP_046218253.1 outer membrane lipoprotein carrier protein LolA -
BEH_RS01410 273582..274739 + 1158 WP_040059883.1 alanine racemase -
BEH_RS01415 275010..275291 + 282 WP_019395219.1 ribbon-helix-helix protein, CopG family Antitoxin
BEH_RS01420 275297..275647 + 351 WP_019395220.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BEH_RS01425 275938..276771 + 834 WP_019395221.1 STAS domain-containing protein -
BEH_RS01430 276774..277130 + 357 WP_019395222.1 STAS domain-containing protein -
BEH_RS01435 277133..277534 + 402 WP_019395223.1 anti-sigma regulatory factor -
BEH_RS01440 277546..278556 + 1011 WP_040059882.1 PP2C family protein-serine/threonine phosphatase -
BEH_RS01445 278614..278946 + 333 WP_019395225.1 STAS domain-containing protein -
BEH_RS01450 278943..279425 + 483 WP_019395226.1 anti-sigma B factor RsbW -
BEH_RS01455 279391..280185 + 795 WP_019395227.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-46)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12919.04 Da        Isoelectric Point: 8.5368

>T55309 WP_019395220.1 NZ_CP011974:275297-275647 [Priestia filamentosa]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKKYGFERDSVILLEQV
RTIDKQRLTDKITHLDEEMMSKVDKALQISLGLIDF

Download         Length: 351 bp

>T55309 NZ_CP011974:275297-275647 [Priestia filamentosa]
TTGGTAGTAAAACGTGGCGACGTTTATTTCGCTGATTTATCTCCCGTTGTTGGTTCTGAGCAAGGAGGGGTCCGACCAGT
CCTTGTTATTCAAAACAACATTGGTAACCGTTTTAGTCCCACGGTTATTGTCGCAGCCATTACCGCTCAAATTCAAAAAG
CAAAGCTACCTACGCATGTAGAAATTAATGCTAAGAAATACGGGTTTGAACGAGACTCCGTTATTCTGTTAGAGCAGGTT
CGAACAATTGATAAGCAGCGTTTAACAGACAAGATTACGCATCTTGATGAAGAAATGATGAGTAAAGTCGATAAAGCGTT
ACAAATTAGCCTTGGGCTTATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10441.78 Da        Isoelectric Point: 5.7202

>AT55309 WP_019395219.1 NZ_CP011974:275010-275291 [Priestia filamentosa]
VSEASATMELLVRLPQHLVSELDAIAKQENSNRNDLINQAVKKFVDEKRNSSIRNNMRRGYMEMAKINLNIASEAFLAEH
EADNTVERLVSGG

Download         Length: 282 bp

>AT55309 NZ_CP011974:275010-275291 [Priestia filamentosa]
GTGTCTGAAGCGAGCGCAACGATGGAGCTTTTAGTTCGTTTACCACAACATTTAGTATCTGAACTAGATGCTATCGCAAA
GCAGGAAAACAGTAACCGAAATGATTTGATTAATCAAGCTGTAAAGAAGTTCGTTGATGAGAAAAGAAATAGTAGCATCC
GCAATAATATGCGACGGGGTTATATGGAAATGGCGAAAATTAACCTAAATATTGCGTCTGAAGCTTTCTTGGCTGAACAT
GAAGCGGACAACACAGTCGAACGTCTTGTAAGTGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A329EQQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A329EU69

References