Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 5179719..5180353 Replicon chromosome
Accession NZ_CP011972
Organism Pseudomonas syringae pv. actinidiae ICMP 18884

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag IYO_RS23305 Protein ID WP_003380365.1
Coordinates 5179949..5180353 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q888H8
Locus tag IYO_RS23300 Protein ID WP_003380366.1
Coordinates 5179719..5179949 (+) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IYO_RS23275 5174783..5175822 + 1040 Protein_4565 TRAP transporter substrate-binding protein -
IYO_RS23280 5175886..5176413 + 528 WP_003380373.1 TRAP transporter small permease -
IYO_RS23285 5176414..5177694 + 1281 WP_003380372.1 TRAP transporter large permease -
IYO_RS23290 5177778..5178748 + 971 Protein_4568 DMT family transporter -
IYO_RS23295 5178723..5179604 + 882 WP_003380368.1 aldose 1-epimerase -
IYO_RS23300 5179719..5179949 + 231 WP_003380366.1 antitoxin Antitoxin
IYO_RS23305 5179949..5180353 + 405 WP_003380365.1 tRNA(fMet)-specific endonuclease VapC Toxin
IYO_RS32290 5180540..5181115 - 576 WP_019334082.1 hypothetical protein -
IYO_RS23310 5181114..5181302 + 189 WP_003380363.1 hypothetical protein -
IYO_RS23315 5181538..5182050 + 513 WP_003380362.1 sigma-70 family RNA polymerase sigma factor -
IYO_RS23320 5182050..5182712 + 663 WP_003380361.1 ChrR family anti-sigma-E factor -
IYO_RS23325 5182788..5183024 - 237 WP_003380360.1 hypothetical protein -
IYO_RS23330 5183089..5184345 - 1257 WP_003380359.1 3-phosphoshikimate 1-carboxyvinyltransferase -
IYO_RS23335 5184441..5184923 - 483 WP_003380358.1 PAS domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-124)

Antitoxin

(7-34)


Sequences


Toxin        


Download         Length: 135 a.a.        Molecular weight: 14814.09 Da        Isoelectric Point: 6.8615

>T55306 WP_003380365.1 NZ_CP011972:5179949-5180353 [Pseudomonas syringae pv. actinidiae ICMP 18884]
MLKYMLDTNICIFTIKNKPVSVREAFNLHHGQLCISAITLMELVYGAEKSSSPERNLAVVEGFAARLELLPYDSAAAAHT
GMIRAELARAGTPIGPYDQMIAGHARSLGLVVITNNQREFQRVEGLRVEDWVSQ

Download         Length: 405 bp

>T55306 NZ_CP011972:5179949-5180353 [Pseudomonas syringae pv. actinidiae ICMP 18884]
ATGCTCAAATACATGCTCGACACCAATATTTGCATCTTCACTATCAAGAACAAGCCGGTGTCGGTACGTGAGGCCTTCAA
CCTTCATCACGGGCAGCTGTGTATCAGCGCGATCACGTTGATGGAGCTTGTTTACGGCGCAGAAAAGTCTTCCAGTCCCG
AAAGAAATCTTGCTGTTGTAGAAGGTTTTGCAGCGCGTCTTGAATTGCTTCCCTACGATAGCGCCGCCGCGGCTCACACC
GGCATGATCAGAGCCGAGCTGGCCAGAGCGGGTACACCGATTGGCCCGTACGATCAAATGATTGCAGGACATGCCCGCTC
TCTTGGCCTCGTGGTCATCACCAACAACCAGCGTGAATTCCAGCGCGTCGAGGGGCTGAGGGTAGAAGACTGGGTAAGCC
AATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8593.67 Da        Isoelectric Point: 4.5557

>AT55306 WP_003380366.1 NZ_CP011972:5179719-5179949 [Pseudomonas syringae pv. actinidiae ICMP 18884]
MEQSTVFKSNRSQAVRLPKAVALPDDVKRVDVVAVGRTRIISPAGEMWNSWFDGESVSDDFMAEREQPVEQLRESL

Download         Length: 231 bp

>AT55306 NZ_CP011972:5179719-5179949 [Pseudomonas syringae pv. actinidiae ICMP 18884]
ATGGAACAATCCACAGTCTTCAAAAGCAATCGAAGTCAGGCTGTTCGACTCCCAAAAGCGGTGGCATTACCCGACGACGT
GAAACGGGTAGACGTCGTAGCAGTGGGCAGGACACGCATCATTTCCCCTGCTGGTGAAATGTGGAACAGTTGGTTCGATG
GTGAAAGTGTCAGTGATGACTTCATGGCAGAGCGCGAGCAGCCAGTTGAACAGCTGCGCGAATCACTCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A0K8LU44

References