Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 2833941..2834502 Replicon chromosome
Accession NZ_CP011971
Organism Steroidobacter denitrificans strain DSM 18526

Toxin (Protein)


Gene name chpK Uniprot ID A0A127FC04
Locus tag ACG33_RS12730 Protein ID WP_066921736.1
Coordinates 2833941..2834279 (-) Length 113 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID A0A127FE12
Locus tag ACG33_RS12735 Protein ID WP_066921738.1
Coordinates 2834266..2834502 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ACG33_RS12710 2828955..2830400 - 1446 WP_157071868.1 DHA2 family efflux MFS transporter permease subunit -
ACG33_RS12715 2830478..2831692 - 1215 WP_066921730.1 efflux RND transporter periplasmic adaptor subunit -
ACG33_RS12720 2831741..2833177 - 1437 WP_066921732.1 efflux transporter outer membrane subunit -
ACG33_RS12725 2833182..2833649 - 468 WP_066921734.1 winged helix DNA-binding protein -
ACG33_RS16380 2833705..2833944 - 240 WP_168160130.1 DUF5343 domain-containing protein -
ACG33_RS12730 2833941..2834279 - 339 WP_066921736.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ACG33_RS12735 2834266..2834502 - 237 WP_066921738.1 hypothetical protein Antitoxin
ACG33_RS16230 2834547..2834684 - 138 WP_157071790.1 hypothetical protein -
ACG33_RS12745 2834801..2835034 + 234 WP_210399066.1 BrnA antitoxin family protein -
ACG33_RS12755 2836290..2837354 - 1065 WP_210399067.1 Fic family protein -
ACG33_RS12760 2837493..2837942 + 450 WP_157071791.1 hypothetical protein -
ACG33_RS12765 2838178..2838798 + 621 WP_210399068.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 12044.97 Da        Isoelectric Point: 11.3689

>T55298 WP_066921736.1 NZ_CP011971:c2834279-2833941 [Steroidobacter denitrificans]
MKRGEVWWASLPTPTGSGPGFRRPVVVVQSNPFNQSRIATVVVAIITSNLALADAPGNLRIGKAESGLAKQSVINVSQLY
TLDRELLTQRVRSLPAEAVRAIDEGLRLVLGL

Download         Length: 339 bp

>T55298 NZ_CP011971:c2834279-2833941 [Steroidobacter denitrificans]
ATGAAACGTGGTGAGGTTTGGTGGGCCTCATTGCCGACTCCCACCGGCTCTGGGCCGGGATTTCGTCGCCCCGTCGTCGT
AGTTCAGTCCAACCCATTTAACCAAAGCCGCATCGCCACAGTCGTTGTGGCGATCATTACTTCCAACCTTGCCTTAGCAG
ATGCTCCTGGAAACCTTCGCATCGGAAAGGCCGAGTCTGGATTGGCCAAACAGTCTGTGATCAACGTATCGCAACTCTAT
ACGCTGGATCGTGAGCTGCTGACCCAACGTGTGCGCTCTCTGCCGGCGGAGGCCGTGCGCGCCATCGACGAAGGCCTGAG
GCTTGTCCTTGGGCTCTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8467.57 Da        Isoelectric Point: 4.9502

>AT55298 WP_066921738.1 NZ_CP011971:c2834502-2834266 [Steroidobacter denitrificans]
MKIAISVPDEVFEAGEHLAQQLGLSRSQLYSDALSAYLSERGAAAVTAKLNAVYATEPSKPDPAFARMQLTRLANETW

Download         Length: 237 bp

>AT55298 NZ_CP011971:c2834502-2834266 [Steroidobacter denitrificans]
ATGAAGATCGCCATCTCCGTTCCAGATGAAGTATTTGAGGCCGGAGAGCATTTGGCACAGCAGCTCGGGTTATCCCGAAG
TCAGCTCTATTCGGATGCCCTATCGGCATACTTGAGCGAACGAGGGGCGGCAGCCGTCACGGCAAAGCTCAACGCGGTAT
ATGCCACGGAACCCTCAAAGCCCGATCCAGCCTTTGCTCGCATGCAACTGACGCGCCTCGCCAATGAAACGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A127FC04

Antitoxin

Source ID Structure
AlphaFold DB A0A127FE12

References