Detailed information of TA system TA055297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 2657780..2658589
• Replicon: chromosome
• Accession: NZ_CP011971
• Organism: Steroidobacter denitrificans strain DSM 18526

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: ACG33_RS12000
• Protein ID: WP_066921491.1
• Coordinates: 2658065..2658589 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A127FDR3
• Locus tag: ACG33_RS11995
• Protein ID: WP_066921489.1
• Coordinates: 2657780..2658064 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACG33_RS11980	2652859..2654022	-	1164	WP_066921483.1	ADP-forming succinate--CoA ligase subunit beta	-
ACG33_RS11985	2654259..2655989	+	1731	WP_066921485.1	HAMP domain-containing histidine kinase	-
ACG33_RS11990	2656004..2657329	+	1326	Protein_2355	sigma-54-dependent Fis family transcriptional regulator	-
ACG33_RS11995	2657780..2658064	+	285	WP_066921489.1	DUF1778 domain-containing protein	Antitoxin
ACG33_RS12000	2658065..2658589	+	525	WP_066921491.1	GNAT family N-acetyltransferase	Toxin
ACG33_RS15825	2658769..2659005	+	237	WP_210399271.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ACG33_RS12010	2659237..2659809	+	573	WP_210399053.1	pilin	-
ACG33_RS12015	2659906..2661102	+	1197	WP_066921495.1	hypothetical protein	-
ACG33_RS12020	2661118..2662323	+	1206	WP_168160085.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (60-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (13-88)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19078.10 Da
• Isoelectric Point: 7.1055

>T55297 WP_066921491.1 NZ_CP011971:2658065-2658589 [Steroidobacter denitrificans]
VPTIPAPGVLDPERLGAHHDLAHFKNGRHESLDAWLRDRALTSEGLSARTYVVCATDTPARLVGYYAISTAMAHRTALPT
AKLRRGMPDEVPLLLIGRLAIDADFQKRGLGVDLLIDALRRCLVAAETAGARGIITHAIDDEAMAFYERHGFVRCPLGER
TMLMPMETARILVD

Nucleotide

• Length: 525 bp

>T55297 NZ_CP011971:2658065-2658589 [Steroidobacter denitrificans]
GTGCCAACGATCCCGGCGCCGGGCGTACTGGACCCCGAGCGCCTTGGCGCTCACCATGATCTTGCGCACTTCAAGAATGG
TAGGCACGAGTCGCTCGATGCATGGCTTCGCGATCGAGCATTGACAAGCGAAGGCCTGTCGGCTCGAACCTATGTCGTGT
GCGCGACCGACACTCCCGCCCGCCTGGTCGGCTATTACGCGATCTCGACCGCAATGGCACATCGAACCGCTCTACCGACG
GCAAAACTCCGTCGCGGCATGCCGGATGAGGTTCCGCTGCTCCTCATCGGTCGATTGGCAATCGATGCCGACTTCCAGAA
GCGCGGATTGGGCGTTGATCTTCTGATCGACGCACTGCGCCGCTGCCTCGTTGCAGCCGAAACGGCTGGAGCCCGAGGGA
TCATCACTCACGCCATCGACGACGAAGCTATGGCGTTCTACGAACGACATGGGTTCGTTCGCTGTCCGCTCGGCGAGCGA
ACGATGCTTATGCCGATGGAGACTGCCCGGATTCTGGTTGATTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10658.17 Da
• Isoelectric Point: 10.2596

>AT55297 WP_066921489.1 NZ_CP011971:2657780-2658064 [Steroidobacter denitrificans]
MSTDSTARKDDLIQIRASATTKATLNRAAAMRGQRLSEFMLESARARAEETILDQRLFALDDDDYKAFLAMLDAPAKPDN
ALRARMKRKPAWER

Nucleotide

• Length: 285 bp

>AT55297 NZ_CP011971:2657780-2658064 [Steroidobacter denitrificans]
ATGAGCACCGATTCGACTGCACGCAAAGACGATCTGATCCAGATCAGAGCCTCTGCTACCACCAAAGCCACCCTCAACCG
AGCGGCGGCCATGCGTGGTCAAAGGCTGTCGGAGTTTATGCTGGAGAGCGCCCGGGCACGAGCTGAGGAGACGATCCTCG
ATCAGCGCTTGTTTGCTCTTGATGACGACGACTACAAGGCATTCCTTGCCATGCTCGATGCTCCGGCCAAGCCAGATAAC
GCACTGCGAGCAAGGATGAAACGCAAGCCGGCGTGGGAACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A127FDR3