Detailed information of TA system TA055293

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 1614912..1615526
• Replicon: chromosome
• Accession: NZ_CP011971
• Organism: Steroidobacter denitrificans strain DSM 18526

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A127F900
• Locus tag: ACG33_RS07310
• Protein ID: WP_066922998.1
• Coordinates: 1615230..1615526 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A127FBD2
• Locus tag: ACG33_RS07305
• Protein ID: WP_066919956.1
• Coordinates: 1614912..1615226 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACG33_RS07275	1609992..1610201	+	210	WP_066919947.1	hypothetical protein	-
ACG33_RS07280	1610202..1610630	-	429	WP_083536537.1	hypothetical protein	-
ACG33_RS07285	1611496..1612323	-	828	WP_066919949.1	hypothetical protein	-
ACG33_RS07290	1612336..1612920	-	585	WP_157071695.1	hypothetical protein	-
ACG33_RS07295	1613052..1613828	-	777	WP_083536538.1	TIGR04255 family protein	-
ACG33_RS07300	1614364..1614708	-	345	WP_066919955.1	tyrosine-type recombinase/integrase	-
ACG33_RS07305	1614912..1615226	-	315	WP_066919956.1	putative addiction module antidote protein	Antitoxin
ACG33_RS07310	1615230..1615526	-	297	WP_066922998.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACG33_RS07315	1615864..1616187	+	324	WP_066919957.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ACG33_RS07320	1616180..1616485	+	306	WP_066919958.1	helix-turn-helix domain-containing protein	-
ACG33_RS07325	1616555..1617061	-	507	WP_157071696.1	hypothetical protein	-
ACG33_RS15680	1617625..1617888	+	264	WP_083537206.1	type II toxin-antitoxin system ParD family antitoxin	-
ACG33_RS15685	1617892..1618182	+	291	WP_083536540.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ACG33_RS07340	1618872..1619960	-	1089	WP_066919979.1	undecaprenyl/decaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-96)

Antitoxin

• Uncharacterized protein HI1420 (7-102)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10801.57 Da
• Isoelectric Point: 10.4000

>T55293 WP_066922998.1 NZ_CP011971:c1615526-1615230 [Steroidobacter denitrificans]
MVELIKTATFDAWLSGLGDLAARARIQLRLDRLALGNPGDVKPVGSGVSELRIDYGPGYRVYYTQRGRVIAVILCGGDKR
TQDKDIKQAIKIAKQWKG

Nucleotide

• Length: 297 bp

>T55293 NZ_CP011971:c1615526-1615230 [Steroidobacter denitrificans]
ATGGTCGAGCTGATCAAAACCGCCACTTTCGATGCCTGGCTTTCAGGCCTGGGAGACCTCGCGGCGCGGGCGCGCATTCA
GCTTCGGCTGGATCGTCTCGCGTTGGGCAATCCCGGTGATGTGAAGCCAGTGGGCTCGGGCGTGAGTGAGTTGCGGATCG
ACTACGGCCCCGGCTACCGGGTGTACTACACACAGCGCGGCCGGGTGATTGCCGTGATCTTGTGCGGCGGCGACAAGCGC
ACGCAGGACAAAGACATCAAGCAGGCGATCAAAATCGCCAAACAGTGGAAGGGTTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 10961.59 Da
• Isoelectric Point: 8.3591

>AT55293 WP_066919956.1 NZ_CP011971:c1615226-1614912 [Steroidobacter denitrificans]
MATKRKTAKETFSRYDTADYLKSEDDIAAYLDAVMEDAADDPALISAALGNVARAYGMIKLANDTGLTREGLYKALGAGG
NPTLATVVKVMKALGMRLSVSKAA

Nucleotide

• Length: 315 bp

>AT55293 NZ_CP011971:c1615226-1614912 [Steroidobacter denitrificans]
ATGGCTACCAAGCGAAAGACTGCAAAGGAGACCTTCTCCCGCTACGACACTGCCGACTACCTCAAGAGCGAAGATGATAT
TGCTGCGTACCTGGACGCTGTCATGGAGGACGCCGCGGACGATCCAGCGCTCATTTCTGCGGCACTCGGCAACGTGGCTC
GCGCGTACGGCATGATCAAGCTCGCCAACGACACCGGCCTTACGCGCGAAGGGCTCTACAAGGCGCTCGGCGCGGGCGGC
AATCCCACGCTTGCAACCGTGGTCAAGGTGATGAAGGCTCTCGGCATGCGGCTGAGCGTGTCGAAAGCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A127F900

Antitoxin

• AlphaFold DB: A0A127FBD2