Detailed information of TA system TA055277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 8589..9130
• Replicon: plasmid unnamed
• Accession: NZ_CP011963
• Organism: Xanthomonas oryzae pv. oryzicola strain CFBP2286

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ACU11_RS21695
• Protein ID: WP_193566048.1
• Coordinates: 8589..8915 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ACU11_RS21700
• Protein ID: WP_048485072.1
• Coordinates: 8912..9130 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACU11_RS21685	3972..5546	+	1575	WP_048485070.1	type IV secretion system DNA-binding domain-containing protein	-
ACU11_RS21690	5560..8520	+	2961	WP_048485071.1	conjugative relaxase	-
ACU11_RS21695	8589..8915	-	327	WP_193566048.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ACU11_RS21700	8912..9130	-	219	WP_048485072.1	antitoxin MazE family protein	Antitoxin
ACU11_RS21705	9349..9768	-	420	WP_048485073.1	hypothetical protein	-
ACU11_RS21710	9894..10802	-	909	WP_048485074.1	lytic transglycosylase domain-containing protein	-
ACU11_RS21715	10789..11811	-	1023	WP_048485075.1	P-type DNA transfer ATPase VirB11	-
ACU11_RS21720	11822..13042	-	1221	WP_048485076.1	TrbI/VirB10 family protein	-
ACU11_RS21725	13044..13826	-	783	WP_048485077.1	P-type conjugative transfer protein VirB9	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..36574	36574

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11646.72 Da
• Isoelectric Point: 10.1008

>T55277 WP_193566048.1 NZ_CP011963:c8915-8589 [Xanthomonas oryzae pv. oryzicola]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVVIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T55277 NZ_CP011963:c8915-8589 [Xanthomonas oryzae pv. oryzicola]
ATGATGCGGGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCACGGCCCGCTCTGGTGATCCAGGCCGA
CCAGTTTGACGCACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTCGTTGCTGCGCCGCTGCTGCGCATTACCG
TTCATCCAAGTACCGACAATGGTTTGCAGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
GTAGGTCGAGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGTAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8360.50 Da
• Isoelectric Point: 6.4486

>AT55277 WP_048485072.1 NZ_CP011963:c9130-8912 [Xanthomonas oryzae pv. oryzicola]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAAECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT55277 NZ_CP011963:c9130-8912 [Xanthomonas oryzae pv. oryzicola]
ATGGCACACGTCAATTCACGAGTTCAAAAGCACCGCGACGCCCTGCGCATGGCAGGGCTGCGTCCGGTGCAAATTTGGGT
GCCGGACACACGGCGGCCTGACTTCGCCGCGGAATGCCGCCGTCAGTGTCGTCTTGCTGCACAAGCGGACATGGCGGACA
CCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCTGGACGGAATGA