Detailed information of TA system TA055249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 19482..20007
• Replicon: plasmid virulence
• Accession: NZ_CP011943
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain OLF-00D989 87-1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: TV13_RS22750
• Protein ID: WP_001159863.1
• Coordinates: 19702..20007 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: TV13_RS22745
• Protein ID: WP_000813641.1
• Coordinates: 19482..19700 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TV13_RS22720	15096..15482	+	387	WP_000751876.1	YgiW/YdeI family stress tolerance OB fold protein	-
TV13_RS24385	15535..15696	+	162	WP_015059604.1	hypothetical protein	-
TV13_RS22725	16023..17012	-	990	WP_000461382.1	RepB family plasmid replication initiator protein	-
TV13_RS24890	17506..17801	-	296	Protein_24	cytoplasmic protein	-
TV13_RS22735	17921..18151	+	231	WP_001057493.1	hypothetical protein	-
TV13_RS22740	18285..18806	-	522	WP_077681952.1	hypothetical protein	-
TV13_RS22745	19482..19700	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
TV13_RS22750	19702..20007	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
TV13_RS22755	20009..20299	+	291	WP_001266176.1	hypothetical protein	-
TV13_RS22760	20296..20817	+	522	WP_000198608.1	hypothetical protein	-
TV13_RS22765	20852..21634	+	783	WP_000082169.1	site-specific integrase	-
TV13_RS22770	21643..22356	+	714	WP_000545756.1	EAL domain-containing protein	-
TV13_RS22775	22360..22869	+	510	WP_001541544.1	CaiF/GrlA family transcriptional regulator	-
TV13_RS22780	22863..23348	+	486	WP_000905606.1	membrane protein	-
TV13_RS24395	23625..23912	-	288	WP_071530243.1	hypothetical protein	-
TV13_RS22790	24068..24628	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..59372	59372

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T55249 WP_001159863.1 NZ_CP011943:19702-20007 [Salmonella enterica subsp. enterica serovar Enteritidis]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T55249 NZ_CP011943:19702-20007 [Salmonella enterica subsp. enterica serovar Enteritidis]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT55249 WP_000813641.1 NZ_CP011943:19482-19700 [Salmonella enterica subsp. enterica serovar Enteritidis]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT55249 NZ_CP011943:19482-19700 [Salmonella enterica subsp. enterica serovar Enteritidis]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6