Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 830880..831513 Replicon chromosome
Accession NZ_CP011939
Organism Weizmannia coagulans strain S-lac

Toxin (Protein)


Gene name mazF Uniprot ID G2TIM0
Locus tag AB434_RS03895 Protein ID WP_013860680.1
Coordinates 830880..831230 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G2TIL9
Locus tag AB434_RS03900 Protein ID WP_014096808.1
Coordinates 831235..831513 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB434_RS03855 826137..826916 - 780 WP_014096815.1 RNA polymerase sigma factor SigB -
AB434_RS03860 826894..827364 - 471 WP_014096814.1 anti-sigma B factor RsbW -
AB434_RS03865 827368..827703 - 336 WP_026104587.1 anti-sigma factor antagonist -
AB434_RS03870 827773..828783 - 1011 WP_017551130.1 PP2C family protein-serine/threonine phosphatase -
AB434_RS03875 828797..829198 - 402 WP_014096811.1 anti-sigma regulatory factor -
AB434_RS03880 829203..829559 - 357 WP_014096810.1 STAS domain-containing protein -
AB434_RS03885 829563..830390 - 828 WP_014096809.1 RsbT co-antagonist protein RsbRA -
AB434_RS03895 830880..831230 - 351 WP_013860680.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AB434_RS03900 831235..831513 - 279 WP_014096808.1 hypothetical protein Antitoxin
AB434_RS03905 831666..832817 - 1152 WP_014096807.1 alanine racemase -
AB434_RS03910 833019..834035 - 1017 WP_035183268.1 outer membrane lipoprotein carrier protein LolA -
AB434_RS03915 834176..834526 - 351 WP_014096805.1 holo-ACP synthase -
AB434_RS03920 834807..835406 + 600 WP_014096804.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12968.01 Da        Isoelectric Point: 4.8998

>T55248 WP_013860680.1 NZ_CP011939:c831230-830880 [Weizmannia coagulans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGLERDSVILLEQI
RTIDKQRLTDKITHLDEEVMEKIDDALQISLGLVEF

Download         Length: 351 bp

>T55248 NZ_CP011939:c831230-830880 [Weizmannia coagulans]
TTGATTGTTAAGCGTGGAGACGTGTACTTCGCCGATCTATCTCCGGTGGTCGGCTCCGAGCAGGGAGGCGTACGTCCTGT
CCTCATTTTACAAAACGACATTGGAAACCGTTTTAGCCCCACTGTCATTGTTGCCGCGATAACAGCGCAAATCCAGAAAG
CAAAATTGCCCACGCATGTGGAAATTGATGCAAAGCGTTACGGTTTGGAAAGGGATTCTGTGATATTGCTTGAACAAATA
CGCACAATCGATAAACAACGCTTAACGGATAAGATTACGCATCTTGATGAAGAAGTCATGGAAAAAATTGACGACGCCCT
GCAAATCAGCCTGGGACTGGTCGAATTTTAG

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10633.19 Da        Isoelectric Point: 5.2204

>AT55248 WP_014096808.1 NZ_CP011939:c831513-831235 [Weizmannia coagulans]
VSEPISTEILVRLPQQLLTELDGFAETENMNRNELIYRATKMYLKERKKRQIRETMRRGYMEMAKINLAIASEAIQAEYE
AEHTVESLVSGG

Download         Length: 279 bp

>AT55248 NZ_CP011939:c831513-831235 [Weizmannia coagulans]
GTGTCTGAACCTATTTCAACAGAAATTTTAGTCCGCTTGCCGCAGCAGCTGTTGACGGAATTGGACGGATTTGCTGAAAC
TGAAAATATGAATCGCAATGAATTAATTTACCGTGCCACAAAAATGTATTTAAAAGAACGGAAAAAACGCCAAATCCGCG
AAACGATGAGGCGTGGTTACATGGAAATGGCAAAAATTAATTTGGCGATTGCCTCCGAAGCGATCCAGGCTGAATACGAA
GCAGAACATACAGTTGAAAGCCTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4BYJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4BX66

References