Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2850335..2850989 Replicon chromosome
Accession NZ_CP011938
Organism Escherichia coli strain C43(DE3)

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag TZ57_RS14130 Protein ID WP_000244783.1
Coordinates 2850335..2850742 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag TZ57_RS14135 Protein ID WP_000354046.1
Coordinates 2850723..2850989 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TZ57_RS14110 2846292..2848025 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
TZ57_RS14115 2848031..2848741 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
TZ57_RS14120 2848766..2849662 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
TZ57_RS14125 2849774..2850295 + 522 WP_001055874.1 flavodoxin FldB -
TZ57_RS14130 2850335..2850742 - 408 WP_000244783.1 protein YgfX Toxin
TZ57_RS14135 2850723..2850989 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
TZ57_RS14140 2851232..2852212 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
TZ57_RS14145 2852408..2853067 - 660 WP_000250274.1 hemolysin III family protein -
TZ57_RS14150 2853231..2853542 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
TZ57_RS14155 2853587..2855020 + 1434 WP_012775976.1 6-phospho-beta-glucosidase BglA -
TZ57_RS14160 2855077..2855820 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T55243 WP_000244783.1 NZ_CP011938:c2850742-2850335 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T55243 NZ_CP011938:c2850742-2850335 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTAGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT55243 WP_000354046.1 NZ_CP011938:c2850989-2850723 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT55243 NZ_CP011938:c2850989-2850723 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References