Detailed information of TA system TA055221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3440532..3441169
• Replicon: chromosome
• Accession: NZ_CP011937
• Organism: Bacillus velezensis strain CBMB205

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: AAV34_RS16955
• Protein ID: WP_003156187.1
• Coordinates: 3440532..3440882 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: AAV34_RS16960
• Protein ID: WP_003156188.1
• Coordinates: 3440888..3441169 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AAV34_RS16915	3435572..3436174	-	603	WP_007410234.1	SpoIIE family protein phosphatase	-
AAV34_RS16920	3436174..3436962	-	789	WP_032873003.1	RNA polymerase sigma factor SigB	-
AAV34_RS16925	3436928..3437410	-	483	WP_007609591.1	anti-sigma B factor RsbW	-
AAV34_RS16930	3437407..3437736	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
AAV34_RS16935	3437800..3438807	-	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
AAV34_RS16940	3438819..3439220	-	402	WP_003156178.1	anti-sigma regulatory factor	-
AAV34_RS16945	3439223..3439588	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
AAV34_RS16950	3439593..3440414	-	822	WP_003156182.1	STAS domain-containing protein	-
AAV34_RS16955	3440532..3440882	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
AAV34_RS16960	3440888..3441169	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
AAV34_RS16965	3441289..3442458	-	1170	WP_032873001.1	alanine racemase	-
AAV34_RS16970	3442575..3443582	-	1008	WP_032872999.1	outer membrane lipoprotein carrier protein LolA	-
AAV34_RS16975	3443747..3444112	-	366	WP_007609580.1	holo-ACP synthase	-
AAV34_RS16980	3444205..3444804	+	600	WP_032872997.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T55221 WP_003156187.1 NZ_CP011937:c3440882-3440532 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T55221 NZ_CP011937:c3440882-3440532 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT55221 WP_003156188.1 NZ_CP011937:c3441169-3440888 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT55221 NZ_CP011937:c3441169-3440888 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5