Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 24258..24829 Replicon plasmid pLIMHP1
Accession NZ_CP011936
Organism Leptospira interrogans serovar Manilae strain UP-MMC-NIID HP

Toxin (Protein)


Gene name mazF Uniprot ID Q8F5A3
Locus tag LIMHP_RS19350 Protein ID WP_000240418.1
Coordinates 24488..24829 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M6U553
Locus tag LIMHP_RS19345 Protein ID WP_000443825.1
Coordinates 24258..24494 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIMHP_RS19315 20487..20642 + 156 WP_017853083.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
LIMHP_RS19320 21247..21450 - 204 WP_000534163.1 hypothetical protein -
LIMHP_RS19325 21511..21888 - 378 WP_001206462.1 hypothetical protein -
LIMHP_RS23005 21893..22169 - 277 Protein_13 BrnT family toxin -
LIMHP_RS19330 22270..22572 + 303 WP_100220184.1 integrase core domain protein -
LIMHP_RS19335 23227..23439 + 213 WP_000447737.1 hypothetical protein -
LIMHP_RS19340 23488..23910 + 423 WP_000402983.1 hypothetical protein -
LIMHP_RS19345 24258..24494 + 237 WP_000443825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LIMHP_RS19350 24488..24829 + 342 WP_000240418.1 endoribonuclease MazF Toxin
LIMHP_RS19355 25193..25426 + 234 WP_000557895.1 hypothetical protein -
LIMHP_RS19370 26886..27473 - 588 WP_000708295.1 hypothetical protein -
LIMHP_RS23015 27639..27824 - 186 WP_000793447.1 hypothetical protein -
LIMHP_RS19375 27836..28300 - 465 WP_000457405.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..70055 70055


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin

Antitoxin PemI-like

(1-78)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12918.12 Da        Isoelectric Point: 9.9595

>T55219 WP_000240418.1 NZ_CP011936:24488-24829 [Leptospira interrogans serovar Manilae]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Download         Length: 342 bp

>T55219 NZ_CP011936:24488-24829 [Leptospira interrogans serovar Manilae]
TTGGTAAAGAATAGAAACTATACTCCTGAAAAAGGAGATATAGTGTGGCTGAACTTTACTCCTCAAGCAGGTCATGAACA
GAAAGGTCGTAGACCTGCACTTGTTTTATCGCCTAAGGAATATAATTCAAAGACAGGGTTAGCAATATTTTGTCCAATTA
CCAGTAAAATTAAAGGTTATCCATTCGAAGTGTTGATTAAATCAAAAAAAATCGATGGTGTAATTCTTTCTGATCAAGTA
AAAAACTTAGATTGGACTATCCGTGAAGCAGAATTTATTGAATCAATAAATAAAGTTTCTTTAAAAGAAGTTTTAGATAA
TATTAAATTATTAATATTCTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8728.95 Da        Isoelectric Point: 5.6663

>AT55219 WP_000443825.1 NZ_CP011936:24258-24494 [Leptospira interrogans serovar Manilae]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Download         Length: 237 bp

>AT55219 NZ_CP011936:24258-24494 [Leptospira interrogans serovar Manilae]
ATGGAATCAATTATTCAAAAATGGGGTAATAGCTTAGGAATTAGAATTCCAAAAGCAATGGCAACGGAATTAGAGTTAAA
TGATGGAAGCCATGTTGAATTACAATATGAAGGAGATAAGATTGTAATATATCCAATGAAAAAAGCTTCATTGGAAGATA
AACTTTCTAAAATTACGAAACAAAATCTTCACTCTGAAATATCTACTGGTAATTCTATAGGCAATGAAGCTTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D8SL30

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HC33

References