Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 1424315..1424891 Replicon chromosome
Accession NZ_CP011934
Organism Leptospira interrogans serovar Manilae strain UP-MMC-NIID HP

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag LIMHP_RS05770 Protein ID WP_000617906.1
Coordinates 1424550..1424891 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag LIMHP_RS05765 Protein ID WP_000844758.1
Coordinates 1424315..1424563 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIMHP_RS23255 1421582..1422041 - 460 Protein_1095 hypothetical protein -
LIMHP_RS05755 1422195..1422491 + 297 WP_000477063.1 helix-turn-helix domain-containing protein -
LIMHP_RS05760 1423210..1424103 + 894 WP_048657678.1 IS982 family transposase -
LIMHP_RS05765 1424315..1424563 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
LIMHP_RS05770 1424550..1424891 + 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LIMHP_RS05775 1425915..1426907 - 993 WP_000041092.1 aldolase -
LIMHP_RS05780 1427047..1427982 - 936 WP_000004530.1 ornithine carbamoyltransferase -
LIMHP_RS05790 1428786..1429253 + 468 WP_000060051.1 hypothetical protein -
LIMHP_RS05795 1429250..1429654 + 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T55218 WP_000617906.1 NZ_CP011934:1424550-1424891 [Leptospira interrogans serovar Manilae]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T55218 NZ_CP011934:1424550-1424891 [Leptospira interrogans serovar Manilae]
ATGATTCGTGGTGAAATTTGGTGGGTAGACTTGGGAATTCCATTTGGAAGTGAGCCTGGATTTAAACGTCCAGTTCTTAT
AATTCAAGATGACTCTTTTAATCAAAGTAATATTAATACAATAGTTTCGATTGCAATTACATCGAATTTAAATCTATCTG
AAGCACCAGGTAATGTTTTCATTAGTAAAAAAGATTCGAGTTTATCAAAGGACTCGGTCATAAATGTCTCTCAAATTGTG
ACTTTAGATAAAGAAAGATTTCTTAACAAAGCGGGTAAACTTAAATCAAATAAATTAGGTGAAGTTGAAATTGGGCTTAA
GTTGGTTACAGGCTTAGATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT55218 WP_000844758.1 NZ_CP011934:1424315-1424563 [Leptospira interrogans serovar Manilae]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT55218 NZ_CP011934:1424315-1424563 [Leptospira interrogans serovar Manilae]
ATGAAGACGGCGATTTCAATTCCTGATGAGTTATTTAGGGCTGCCGAGAAAATAGCTAAAAAACTTGGGATTCCTCGAAG
CCAGTTATTTGCAAAAGCATTAGAAGAATTTATCCAATCTCATAGTAAAGAATCTGTAACTGAGAAATTAAATAAAATCT
ACAGTAATAAATCCAAAGAAACTAGAAATAATATTACTGACTTATCCGTAGAATCGCTTCGTAAGAGTTTAAAAAATGAT
TCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References