Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 1017593..1018221 Replicon chromosome
Accession NZ_CP011934
Organism Leptospira interrogans serovar Manilae strain UP-MMC-NIID HP

Toxin (Protein)


Gene name vapC Uniprot ID Q8F7E4
Locus tag LIMHP_RS04160 Protein ID WP_000281042.1
Coordinates 1017593..1017991 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A2H1XEF7
Locus tag LIMHP_RS04165 Protein ID WP_001192480.1
Coordinates 1017991..1018221 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIMHP_RS04135 1012906..1013673 + 768 WP_000636951.1 DUF1554 domain-containing protein -
LIMHP_RS04140 1014192..1015052 + 861 WP_000682878.1 hypothetical protein -
LIMHP_RS04145 1015269..1015727 - 459 WP_001239002.1 GNAT family N-acetyltransferase -
LIMHP_RS04150 1015728..1016804 - 1077 WP_001170579.1 hypothetical protein -
LIMHP_RS04155 1016947..1017495 - 549 WP_000479571.1 hypothetical protein -
LIMHP_RS04160 1017593..1017991 - 399 WP_000281042.1 type II toxin-antitoxin system VapC family toxin Toxin
LIMHP_RS04165 1017991..1018221 - 231 WP_001192480.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LIMHP_RS04170 1018501..1019880 - 1380 WP_000154202.1 DUF4041 domain-containing protein -
LIMHP_RS04175 1020076..1020492 - 417 WP_000799840.1 PIN domain-containing protein -
LIMHP_RS04180 1020470..1020730 - 261 WP_033108532.1 ribbon-helix-helix domain-containing protein -
LIMHP_RS04185 1020942..1021676 - 735 WP_001191971.1 hypothetical protein -
LIMHP_RS04190 1021800..1023080 - 1281 WP_000861193.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15139.76 Da        Isoelectric Point: 9.6893

>T55217 WP_000281042.1 NZ_CP011934:c1017991-1017593 [Leptospira interrogans serovar Manilae]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Download         Length: 399 bp

>T55217 NZ_CP011934:c1017991-1017593 [Leptospira interrogans serovar Manilae]
ATGTATCTTTTGGATACCAATATTTGTATTTTTCTAATTAAAAAGAAAAATGCGACTCTGTTAGAAAATCTTAAGAAAAA
ATTAAATAAAGATCTATTTGTTTCGTCTTTAACTGTTGCGGAACTTGAGTTTGGAATTCAGAAAAGCGAATTTAAAGAAA
AGAATAAAGTTGCACTAATCGAATTTTTAACGATTTTCAATATACTTTCCTTTTCTGATAAAGATGCAGAGTCTTACGGT
ATCATCAGAGCGGATTTGGAGAGGAAAGGTAATGTAATCGGTTCAATAGACATGTTACTTGCCGCTCAAGCAATTGCTAA
TAATTATATCTTTGTTACTAATAATACAAAAGAATTTAAAAGAATTAAGGCTTTAAAAATAGAAAATTGGACACAGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8729.06 Da        Isoelectric Point: 9.8940

>AT55217 WP_001192480.1 NZ_CP011934:c1018221-1017991 [Leptospira interrogans serovar Manilae]
MQTAKLFINGRSQAVRLPKEFQFAGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEGREQPKFDKREKL

Download         Length: 231 bp

>AT55217 NZ_CP011934:c1018221-1017991 [Leptospira interrogans serovar Manilae]
ATGCAAACAGCCAAATTATTTATAAATGGAAGAAGTCAAGCGGTTCGACTACCAAAGGAATTTCAATTTGCCGGAGATGA
TGTTCTCATTCAAAAAGTCGGAGAAGCTGTAATATTGGTTCCGAAAAACAAGGCCTGGAACGTATTTTTAGAAGGATTGA
ACGGGTTTTCAGATGATTTCTTTAAGGAGGGGAGAGAACAACCGAAATTCGATAAAAGAGAAAAACTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6HAN6

Antitoxin

Source ID Structure
AlphaFold DB A0A2H1XEF7

References