55215

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 1424313..1424889 Replicon chromosome
Accession NZ_CP011931
Organism Leptospira interrogans serovar Manilae strain UP-MMC-NIID LP

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag LIMLP_RS05780 Protein ID WP_000617906.1
Coordinates 1424548..1424889 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag LIMLP_RS05775 Protein ID WP_000844758.1
Coordinates 1424313..1424561 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIMLP_RS23270 1421580..1422039 - 460 Protein_1095 hypothetical protein -
LIMLP_RS05765 1422193..1422489 + 297 WP_000477063.1 helix-turn-helix domain-containing protein -
LIMLP_RS05770 1423208..1424101 + 894 WP_048657678.1 IS982 family transposase -
LIMLP_RS05775 1424313..1424561 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
LIMLP_RS05780 1424548..1424889 + 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LIMLP_RS05785 1425913..1426905 - 993 WP_000041092.1 aldolase -
LIMLP_RS05790 1427045..1427980 - 936 WP_000004530.1 ornithine carbamoyltransferase -
LIMLP_RS05800 1428784..1429251 + 468 WP_000060051.1 hypothetical protein -
LIMLP_RS05805 1429248..1429652 + 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T55215 WP_000617906.1 NZ_CP011931:1424548-1424889 [Leptospira interrogans serovar Manilae]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T55215 NZ_CP011931:1424548-1424889 [Leptospira interrogans serovar Manilae]
ATGATTCGTGGTGAAATTTGGTGGGTAGACTTGGGAATTCCATTTGGAAGTGAGCCTGGATTTAAACGTCCAGTTCTTAT
AATTCAAGATGACTCTTTTAATCAAAGTAATATTAATACAATAGTTTCGATTGCAATTACATCGAATTTAAATCTATCTG
AAGCACCAGGTAATGTTTTCATTAGTAAAAAAGATTCGAGTTTATCAAAGGACTCGGTCATAAATGTCTCTCAAATTGTG
ACTTTAGATAAAGAAAGATTTCTTAACAAAGCGGGTAAACTTAAATCAAATAAATTAGGTGAAGTTGAAATTGGGCTTAA
GTTGGTTACAGGCTTAGATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT55215 WP_000844758.1 NZ_CP011931:1424313-1424561 [Leptospira interrogans serovar Manilae]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT55215 NZ_CP011931:1424313-1424561 [Leptospira interrogans serovar Manilae]
ATGAAGACGGCGATTTCAATTCCTGATGAGTTATTTAGGGCTGCCGAGAAAATAGCTAAAAAACTTGGGATTCCTCGAAG
CCAGTTATTTGCAAAAGCATTAGAAGAATTTATCCAATCTCATAGTAAAGAATCTGTAACTGAGAAATTAAATAAAATCT
ACAGTAATAAATCCAAAGAAACTAGAAATAATATTACTGACTTATCCGTAGAATCGCTTCGTAAGAGTTTAAAAAATGAT
TCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References