Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 126344..126938 Replicon plasmid pBCEN1232
Accession NZ_CP011920
Organism Burkholderia cenocepacia strain ST32 isolate 1232

Toxin (Protein)


Gene name PumA Uniprot ID A0A6P2MZL0
Locus tag TQ36_RS35915 Protein ID WP_058904084.1
Coordinates 126645..126938 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A4S3HA87
Locus tag TQ36_RS35910 Protein ID WP_011695198.1
Coordinates 126344..126643 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TQ36_RS35895 121593..123710 - 2118 WP_058904081.1 type IV secretion system DNA-binding domain-containing protein -
TQ36_RS35900 123703..124104 - 402 WP_058904082.1 hypothetical protein -
TQ36_RS35905 125868..126179 + 312 WP_058904083.1 hypothetical protein -
TQ36_RS35910 126344..126643 - 300 WP_011695198.1 putative addiction module antidote protein Antitoxin
TQ36_RS35915 126645..126938 - 294 WP_058904084.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
TQ36_RS38930 127042..127185 - 144 WP_154641514.1 hypothetical protein -
TQ36_RS35920 127243..127464 - 222 WP_058904085.1 DUF3717 domain-containing protein -
TQ36_RS35925 127509..128060 - 552 WP_077021560.1 hypothetical protein -
TQ36_RS35930 128035..128301 - 267 WP_011695194.1 hypothetical protein -
TQ36_RS38400 128316..128711 - 396 WP_011695193.1 hypothetical protein -
TQ36_RS35935 129344..131473 - 2130 WP_058904087.1 ParB/RepB/Spo0J family partition protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..191945 191945


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(11-87)

Antitoxin

Uncharacterized protein HI1420

(4-95)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10870.44 Da        Isoelectric Point: 10.3398

>T55204 WP_058904084.1 NZ_CP011920:c126938-126645 [Burkholderia cenocepacia]
MRIEQTDDFAKWLRGLRDHIARAQIAKRIQRLARGQYGDVKSVGDGVSEMRVHVGPGYRVYFVQRGSTLVILLCGGDKST
QQRDIERAIALSAQLGD

Download         Length: 294 bp

>T55204 NZ_CP011920:c126938-126645 [Burkholderia cenocepacia]
ATGAGAATCGAGCAGACAGACGACTTCGCGAAATGGCTGCGCGGCCTACGTGACCACATCGCGCGCGCGCAGATCGCCAA
GCGGATTCAACGGCTCGCGCGCGGCCAGTACGGGGACGTGAAGTCGGTGGGTGACGGGGTGAGCGAAATGCGCGTGCACG
TCGGCCCCGGCTATCGCGTCTACTTTGTCCAACGAGGCTCAACGCTCGTCATCCTGCTATGCGGTGGCGACAAGTCAACG
CAGCAGCGCGACATTGAGCGCGCTATCGCGCTTTCGGCGCAATTGGGAGATTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10701.99 Da        Isoelectric Point: 4.6364

>AT55204 WP_011695198.1 NZ_CP011920:c126643-126344 [Burkholderia cenocepacia]
MGIKTTPFDAAEYLDTPEAQAEYLRVSFETGDSADIQEAIGTVARARGMTQIARDAGVGRESLYKALDEHGNPEFATILR
VVRALGLTLTVTTPVHEHA

Download         Length: 300 bp

>AT55204 NZ_CP011920:c126643-126344 [Burkholderia cenocepacia]
ATGGGTATCAAGACAACGCCGTTTGACGCGGCCGAATACCTCGACACGCCCGAGGCGCAGGCCGAATATCTGCGGGTTTC
GTTCGAGACTGGCGATTCTGCCGACATTCAGGAAGCCATCGGCACTGTGGCGCGTGCGCGCGGCATGACGCAGATTGCGC
GCGACGCGGGCGTTGGCCGCGAAAGCCTCTACAAGGCGCTGGACGAGCACGGCAACCCGGAATTCGCGACGATCCTGCGT
GTGGTGCGCGCGCTCGGCCTCACTTTGACGGTGACGACGCCTGTACACGAGCACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6P2MZL0

Antitoxin

Source ID Structure
AlphaFold DB A0A4S3HA87

References