Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 29530..30179 Replicon plasmid pBCEN1232
Accession NZ_CP011920
Organism Burkholderia cenocepacia strain ST32 isolate 1232

Toxin (Protein)


Gene name vapC Uniprot ID A0A6P2QTM6
Locus tag TQ36_RS35470 Protein ID WP_058904014.1
Coordinates 29530..29940 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6P2QTN4
Locus tag TQ36_RS35475 Protein ID WP_058904127.1
Coordinates 29937..30179 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TQ36_RS35430 24600..24998 - 399 WP_077175733.1 hypothetical protein -
TQ36_RS35435 25004..26089 - 1086 WP_058904007.1 hypothetical protein -
TQ36_RS35440 26117..26458 - 342 WP_058904008.1 hypothetical protein -
TQ36_RS35445 26744..27253 - 510 WP_058904009.1 hypothetical protein -
TQ36_RS35450 27277..27483 - 207 WP_058904010.1 hypothetical protein -
TQ36_RS35455 27510..28208 - 699 WP_058904011.1 hypothetical protein -
TQ36_RS35460 28394..28723 - 330 WP_058904012.1 hypothetical protein -
TQ36_RS35465 28871..29218 - 348 WP_058904013.1 hypothetical protein -
TQ36_RS35470 29530..29940 - 411 WP_058904014.1 type II toxin-antitoxin system VapC family toxin Toxin
TQ36_RS35475 29937..30179 - 243 WP_058904127.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
TQ36_RS35480 30297..31328 - 1032 WP_058904015.1 non-homologous end-joining DNA ligase -
TQ36_RS35485 31784..32455 + 672 WP_058904016.1 AAA family ATPase -
TQ36_RS35490 32455..33420 + 966 WP_058904017.1 ParB/RepB/Spo0J family partition protein -
TQ36_RS35495 34165..35022 + 858 WP_058904018.1 replication initiator protein A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..191945 191945


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-125)

Antitoxin

SpoVT-AbrB domain

(12-50)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14726.98 Da        Isoelectric Point: 5.1526

>T55203 WP_058904014.1 NZ_CP011920:c29940-29530 [Burkholderia cenocepacia]
MTLYLLDTNILSNVIRDPRGACAARIGETPPEQVCTSIIVAAELRFGVWKRGSSALAQRVEQLLASLTVLPLQPDADRCY
GRLRAELEKQGQLIGANDMLIAAHALAVDAVLVTDNTAEFTRVAGLPVENWLRSAT

Download         Length: 411 bp

>T55203 NZ_CP011920:c29940-29530 [Burkholderia cenocepacia]
ATGACGCTGTATCTGCTCGACACCAATATCCTGTCGAACGTGATCCGCGACCCGCGCGGCGCATGCGCCGCCCGGATCGG
AGAAACTCCGCCGGAGCAAGTGTGCACCTCGATCATCGTCGCCGCCGAGTTACGGTTCGGCGTCTGGAAGCGTGGCTCGT
CAGCGCTCGCGCAGCGCGTCGAGCAGCTGCTCGCAAGCCTGACCGTGTTGCCGCTGCAGCCCGACGCGGACCGGTGCTAC
GGGCGGTTGCGGGCCGAGCTCGAGAAGCAAGGCCAGCTGATCGGCGCCAACGACATGCTGATCGCCGCGCATGCGTTGGC
CGTCGACGCGGTCCTGGTCACGGACAACACGGCTGAATTCACCCGGGTCGCCGGGCTGCCGGTCGAAAACTGGCTGCGAT
CGGCGACGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9324.55 Da        Isoelectric Point: 4.6856

>AT55203 WP_058904127.1 NZ_CP011920:c30179-29937 [Burkholderia cenocepacia]
MPTHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAAPLRELFAQWTPLDETFPDIEDLPAEPVDIE

Download         Length: 243 bp

>AT55203 NZ_CP011920:c30179-29937 [Burkholderia cenocepacia]
ATGCCCACCCATGACACCCGCCACGCGCGCCTGTTTCGCAACGGGCGAAGCCAGGCCGTGCGGATCCCGCGCGAATTCGA
ACTGCCGGTTTCGGAGGTCACCATCCACCGGGAAGGCCGCCGCTTGATCATTGAGCCCGTAGAGGCCGCGCCCTTGCGCG
AGCTGTTCGCCCAATGGACCCCGCTGGACGAGACCTTTCCGGACATCGAGGACCTTCCGGCTGAACCGGTCGACATCGAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6P2QTM6

Antitoxin

Source ID Structure
AlphaFold DB A0A6P2QTN4

References