Detailed information of TA system TA055195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3347698..3348740
• Replicon: chromosome
• Accession: NZ_CP011917
• Organism: Burkholderia cenocepacia strain ST32 isolate 1232

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: TQ36_RS15305
• Protein ID: WP_003153636.1
• Coordinates: 3348165..3348740 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: TQ36_RS15300
• Protein ID: WP_003050245.1
• Coordinates: 3347698..3348168 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TQ36_RS15270	3343088..3344506	-	1419	WP_004350605.1	TIGR03752 family integrating conjugative element protein	-
TQ36_RS15275	3344496..3345407	-	912	WP_004350604.1	TIGR03749 family integrating conjugative element protein	-
TQ36_RS15280	3345404..3346096	-	693	WP_004350603.1	TIGR03746 family integrating conjugative element protein	-
TQ36_RS15285	3346093..3346491	-	399	WP_003153632.1	TIGR03750 family conjugal transfer protein	-
TQ36_RS15290	3346504..3346863	-	360	WP_003153634.1	TIGR03745 family integrating conjugative element membrane protein	-
TQ36_RS37115	3346880..3347113	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
TQ36_RS15295	3347117..3347494	-	378	Protein_3053	RAQPRD family integrative conjugative element protein	-
TQ36_RS15300	3347698..3348168	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
TQ36_RS15305	3348165..3348740	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
TQ36_RS15310	3348758..3349672	+	915	WP_003050256.1	AAA family ATPase	-
TQ36_RS15315	3349669..3350139	+	471	WP_003153638.1	hypothetical protein	-
TQ36_RS15320	3350136..3350636	+	501	WP_003090159.1	hypothetical protein	-
TQ36_RS15325	3350636..3351331	+	696	Protein_3059	nucleotidyltransferase domain-containing protein	-
TQ36_RS15330	3351374..3352552	-	1179	WP_105773736.1	IS256 family transposase	-
TQ36_RS38610	3352701..3352916	+	216	Protein_3061	nucleotidyltransferase	-
TQ36_RS15335	3352955..3353680	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T55195 WP_003153636.1 NZ_CP011917:3348165-3348740 [Burkholderia cenocepacia]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T55195 NZ_CP011917:3348165-3348740 [Burkholderia cenocepacia]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAATCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCGGGCCTGACATTACCCGATCCGAACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT55195 WP_003050245.1 NZ_CP011917:3347698-3348168 [Burkholderia cenocepacia]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT55195 NZ_CP011917:3347698-3348168 [Burkholderia cenocepacia]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68