Detailed information of TA system TA055193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 82613..83160
• Replicon: plasmid pPSUO2
• Accession: NZ_CP011916
• Organism: Escherichia coli strain PSUO2

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: ACJ74_RS25750
• Protein ID: WP_000969996.1
• Coordinates: 82613..82894 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A3A1Q2G3
• Locus tag: ACJ74_RS25755
• Protein ID: WP_042021804.1
• Coordinates: 82891..83160 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACJ74_RS25715	77716..78642	+	927	WP_080077783.1	hypothetical protein	-
ACJ74_RS25720	78692..78946	+	255	WP_059291112.1	hypothetical protein	-
ACJ74_RS25725	79444..79788	+	345	WP_087601730.1	abortive infection protein	-
ACJ74_RS25730	79962..80963	-	1002	WP_000774874.1	DUF4238 domain-containing protein	-
ACJ74_RS25735	81314..81571	+	258	WP_000371888.1	hypothetical protein	-
ACJ74_RS25740	81571..82161	+	591	WP_000194553.1	hypothetical protein	-
ACJ74_RS25745	82181..82528	-	348	WP_024148462.1	hypothetical protein	-
ACJ74_RS25750	82613..82894	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACJ74_RS25755	82891..83160	-	270	WP_042021804.1	hypothetical protein	Antitoxin
ACJ74_RS25770	84076..84933	-	858	WP_001697864.1	incFII family plasmid replication initiator RepA	-
ACJ74_RS25775	84926..85000	-	75	WP_001375168.1	RepA leader peptide Tap	-
ACJ74_RS25785	85236..85493	-	258	WP_000083826.1	replication regulatory protein RepA	-
ACJ74_RS25790	85733..86323	-	591	WP_000766805.1	DUF2726 domain-containing protein	-
ACJ74_RS25795	86361..86570	-	210	WP_001298565.1	hemolysin expression modulator Hha	-
ACJ74_RS25800	86616..87077	-	462	WP_001233838.1	thermonuclease family protein	-
ACJ74_RS25805	87322..87534	-	213	WP_001309245.1	ANR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN	1..110961	110961

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T55193 WP_000969996.1 NZ_CP011916:c82894-82613 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T55193 NZ_CP011916:c82894-82613 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10122.48 Da
• Isoelectric Point: 10.2879

>AT55193 WP_042021804.1 NZ_CP011916:c83160-82891 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLGRGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT55193 NZ_CP011916:c83160-82891 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATACGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGGGCGCGGCGATGTACTTCCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: A0A3A1Q2G3