Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2846880..2847470 Replicon chromosome
Accession NZ_CP011915
Organism Escherichia coli strain PSUO2

Toxin (Protein)


Gene name pemK Uniprot ID V0VE57
Locus tag ACJ74_RS14190 Protein ID WP_000373724.1
Coordinates 2847138..2847470 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag ACJ74_RS14185 Protein ID WP_000288812.1
Coordinates 2846880..2847137 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ACJ74_RS14165 2844493..2845629 + 1137 WP_000420971.1 ISAs1 family transposase -
ACJ74_RS14170 2845862..2846329 + 468 WP_000201267.1 hypothetical protein -
ACJ74_RS14175 2846326..2846532 + 207 WP_000983730.1 helix-turn-helix domain-containing protein -
ACJ74_RS14180 2846591..2846782 - 192 WP_077561361.1 hypothetical protein -
ACJ74_RS14185 2846880..2847137 + 258 WP_000288812.1 antitoxin Antitoxin
ACJ74_RS14190 2847138..2847470 + 333 WP_000373724.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ACJ74_RS14195 2847517..2847873 - 357 WP_001147230.1 hypothetical protein -
ACJ74_RS14200 2847854..2848180 - 327 Protein_2606 hypothetical protein -
ACJ74_RS14205 2848318..2849196 + 879 WP_000511773.1 integrase domain-containing protein -
ACJ74_RS14220 2850148..2850606 + 459 WP_157673257.1 IS200/IS605 family transposase -
ACJ74_RS14225 2850745..2852007 - 1263 WP_001609141.1 integrase arm-type DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2817747..2868519 50772
- inside IScluster/Tn - - 2844493..2850606 6113


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11724.57 Da        Isoelectric Point: 10.5834

>T55172 WP_000373724.1 NZ_CP011915:2847138-2847470 [Escherichia coli]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

>T55172 NZ_CP011915:2847138-2847470 [Escherichia coli]
ATGGACAGAGGGGAAATCTGGCTGGTTTCACTGGATCCGATTGCAGGTCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCCGTTGTTGTTCCCGTTACCAGCGGCGGAAACTTCGCCCGTG
CAGCCGGTTTTACCGTCTCACTGGACGGTGCGGGAACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGAGCCCGGAGCGGTAAGCGACTGGAACGTATACCCGATGCTGTCGTGAATGAAGTGCTGGCCCGACTGGAGGC
CATTCTGAGCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT55172 WP_000288812.1 NZ_CP011915:2846880-2847137 [Escherichia coli]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT55172 NZ_CP011915:2846880-2847137 [Escherichia coli]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCCCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGTTGTTGGCGCAGTGCGATCCGCACGCAGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VE57

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References