55170

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2007533..2008116 Replicon chromosome
Accession NZ_CP011915
Organism Escherichia coli strain PSUO2

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag ACJ74_RS10075 Protein ID WP_000254750.1
Coordinates 2007781..2008116 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag ACJ74_RS10070 Protein ID WP_000581937.1
Coordinates 2007533..2007781 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ACJ74_RS10060 2003872..2005173 + 1302 WP_000046817.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
ACJ74_RS10065 2005221..2007455 + 2235 WP_000226815.1 GTP pyrophosphokinase -
ACJ74_RS10070 2007533..2007781 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
ACJ74_RS10075 2007781..2008116 + 336 WP_000254750.1 endoribonuclease MazF Toxin
ACJ74_RS10080 2008188..2008979 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
ACJ74_RS10085 2009207..2010844 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
ACJ74_RS10090 2010932..2012230 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T55170 WP_000254750.1 NZ_CP011915:2007781-2008116 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T55170 NZ_CP011915:2007781-2008116 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT55170 WP_000581937.1 NZ_CP011915:2007533-2007781 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT55170 NZ_CP011915:2007533-2007781 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LMB4

References