Detailed information of TA system TA055168

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1675421..1676253
• Replicon: chromosome
• Accession: NZ_CP011915
• Organism: Escherichia coli strain PSUO2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: ACJ74_RS08490
• Protein ID: WP_000854753.1
• Coordinates: 1675421..1675795 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: ACJ74_RS08495
• Protein ID: WP_001278232.1
• Coordinates: 1675885..1676253 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACJ74_RS08460	1670535..1671683	-	1149	WP_000905922.1	capsule polysaccharide export inner-membrane protein KpsE	-
ACJ74_RS08465	1671755..1672738	-	984	WP_001331698.1	KpsF/GutQ family sugar-phosphate isomerase	-
ACJ74_RS08470	1673549..1673719	-	171	Protein_1542	IS110 family transposase	-
ACJ74_RS08475	1674061..1674630	-	570	WP_001290241.1	DUF4942 domain-containing protein	-
ACJ74_RS08480	1674727..1674924	-	198	WP_000839281.1	DUF957 domain-containing protein	-
ACJ74_RS08485	1674936..1675424	-	489	WP_000777541.1	hypothetical protein	-
ACJ74_RS08490	1675421..1675795	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
ACJ74_RS08495	1675885..1676253	-	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
ACJ74_RS08505	1676416..1676637	-	222	WP_000692316.1	DUF987 domain-containing protein	-
ACJ74_RS08510	1676700..1677176	-	477	WP_001186726.1	RadC family protein	-
ACJ74_RS08515	1677192..1677677	-	486	WP_001520165.1	antirestriction protein	-
ACJ74_RS08520	1677732..1678550	-	819	WP_001520163.1	DUF945 domain-containing protein	-
ACJ74_RS08530	1678650..1678883	-	234	WP_001119729.1	DUF905 family protein	-
ACJ74_RS08535	1678962..1679417	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T55168 WP_000854753.1 NZ_CP011915:c1675795-1675421 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T55168 NZ_CP011915:c1675795-1675421 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCCACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT55168 WP_001278232.1 NZ_CP011915:c1676253-1675885 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT55168 NZ_CP011915:c1676253-1675885 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: E3PJ72