Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1471285..1471921 Replicon chromosome
Accession NZ_CP011882
Organism Bacillus subtilis strain TO-A JPC

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ABU16_RS07500 Protein ID WP_003156187.1
Coordinates 1471571..1471921 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag ABU16_RS07495 Protein ID WP_003225183.1
Coordinates 1471285..1471566 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ABU16_RS07475 1467644..1468243 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
ABU16_RS07480 1468338..1468703 + 366 WP_003234281.1 holo-ACP synthase -
ABU16_RS07485 1468869..1469885 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
ABU16_RS07490 1470000..1471169 + 1170 WP_003234284.1 alanine racemase -
ABU16_RS07495 1471285..1471566 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ABU16_RS07500 1471571..1471921 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ABU16_RS07505 1472036..1472860 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
ABU16_RS07510 1472865..1473230 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
ABU16_RS07515 1473234..1473635 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
ABU16_RS07520 1473647..1474654 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
ABU16_RS07525 1474716..1475045 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
ABU16_RS07530 1475042..1475524 + 483 WP_003234299.1 anti-sigma B factor RsbW -
ABU16_RS07535 1475490..1476278 + 789 WP_048217624.1 RNA polymerase sigma factor SigB -
ABU16_RS07540 1476278..1476877 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T55144 WP_003156187.1 NZ_CP011882:1471571-1471921 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T55144 NZ_CP011882:1471571-1471921 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT55144 WP_003225183.1 NZ_CP011882:1471285-1471566 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT55144 NZ_CP011882:1471285-1471566 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References