Detailed information of TA system TA055144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1471285..1471921
• Replicon: chromosome
• Accession: NZ_CP011882
• Organism: Bacillus subtilis strain TO-A JPC

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: ABU16_RS07500
• Protein ID: WP_003156187.1
• Coordinates: 1471571..1471921 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: ABU16_RS07495
• Protein ID: WP_003225183.1
• Coordinates: 1471285..1471566 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABU16_RS07475	1467644..1468243	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
ABU16_RS07480	1468338..1468703	+	366	WP_003234281.1	holo-ACP synthase	-
ABU16_RS07485	1468869..1469885	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
ABU16_RS07490	1470000..1471169	+	1170	WP_003234284.1	alanine racemase	-
ABU16_RS07495	1471285..1471566	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
ABU16_RS07500	1471571..1471921	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
ABU16_RS07505	1472036..1472860	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
ABU16_RS07510	1472865..1473230	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
ABU16_RS07515	1473234..1473635	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
ABU16_RS07520	1473647..1474654	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
ABU16_RS07525	1474716..1475045	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
ABU16_RS07530	1475042..1475524	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
ABU16_RS07535	1475490..1476278	+	789	WP_048217624.1	RNA polymerase sigma factor SigB	-
ABU16_RS07540	1476278..1476877	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T55144 WP_003156187.1 NZ_CP011882:1471571-1471921 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T55144 NZ_CP011882:1471571-1471921 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT55144 WP_003225183.1 NZ_CP011882:1471285-1471566 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT55144 NZ_CP011882:1471285-1471566 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32