Detailed information of TA system TA055141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 3615997..3616613
• Replicon: chromosome
• Accession: NZ_CP011863
• Organism: Enterobacter asburiae strain ATCC 35953

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ACJ69_RS17475
• Protein ID: WP_081051446.1
• Coordinates: 3616242..3616613 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A376FEL2
• Locus tag: ACJ69_RS17470
• Protein ID: WP_029741590.1
• Coordinates: 3615997..3616239 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACJ69_RS17440	3611415..3611885	+	471	WP_054829776.1	hypothetical protein	-
ACJ69_RS17445	3611974..3612573	+	600	WP_029740331.1	glucose-1-phosphatase	-
ACJ69_RS17450	3612567..3613448	+	882	WP_047646947.1	virulence factor BrkB family protein	-
ACJ69_RS17455	3613445..3613882	+	438	WP_008501836.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ACJ69_RS17460	3613927..3614868	+	942	WP_059347397.1	fatty acid biosynthesis protein FabY	-
ACJ69_RS17465	3614877..3615797	-	921	WP_054829775.1	alpha/beta hydrolase	-
ACJ69_RS17470	3615997..3616239	+	243	WP_029741590.1	ribbon-helix-helix domain-containing protein	Antitoxin
ACJ69_RS17475	3616242..3616613	+	372	WP_081051446.1	type II toxin-antitoxin system VapC family toxin	Toxin
ACJ69_RS17480	3616653..3617582	-	930	WP_029741588.1	formate dehydrogenase accessory protein FdhE	-
ACJ69_RS17485	3617579..3618214	-	636	WP_010436852.1	formate dehydrogenase cytochrome b556 subunit	-
ACJ69_RS17490	3618211..3619113	-	903	WP_008501831.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13589.71 Da
• Isoelectric Point: 6.9789

>T55141 WP_081051446.1 NZ_CP011863:3616242-3616613 [Enterobacter asburiae]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTAAVMGAFEIIDISRDIAERSVLLR
EKHGMKLPDAIILATAQSRSCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T55141 NZ_CP011863:3616242-3616613 [Enterobacter asburiae]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATCGATCTTTTTAATAATCGTGTTGAGGCGGCGGATGCCATTGA
TCGCACGGCGTCGCATAGAGCCATTAGCCTGATCACGTGGATGGAGGTCATGGTGGGTGCGCGCAAGCACGGACATGAAG
CAAAAACGGCGGCTGTGATGGGGGCTTTTGAGATTATTGATATTTCTCGGGATATCGCAGAAAGAAGCGTTTTACTTCGC
GAGAAACATGGGATGAAACTGCCTGATGCCATTATTCTGGCTACTGCTCAGAGCAGAAGTTGCCCGTTAATCTCACGCAA
TACGAAAGACTTTTCGGGCATTACTGGAGTCGTTTCACCTTACCAGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9401.51 Da
• Isoelectric Point: 4.1735

>AT55141 WP_029741590.1 NZ_CP011863:3615997-3616239 [Enterobacter asburiae]
MSLSADLDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT55141 NZ_CP011863:3615997-3616239 [Enterobacter asburiae]
ATGAGTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGATGACGTAATTCAACGACTGGATAATCT
GAAGCAGCTGCGTAACCAACCTCGTGCCGAATTACTGCGTGAAGCAATTGAGCAATATCTCGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCCTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A376FEL2