Detailed information of TA system TA055088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 74823..75706
• Replicon: plasmid pacX50fB3
• Accession: NZ_CP011836
• Organism: Azotobacter chroococcum strain B3

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: ACG10_RS21675
• Protein ID: WP_089169992.1
• Coordinates: 75269..75706 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: PrcA
• Uniprot ID: -
• Locus tag: ACG10_RS21670
• Protein ID: WP_089169991.1
• Coordinates: 74823..75272 (+)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACG10_RS21645	70313..70954	+	642	WP_089169662.1	cysteine hydrolase	-
ACG10_RS21650	71019..72074	+	1056	WP_089169990.1	aldo/keto reductase	-
ACG10_RS21655	72132..73232	+	1101	WP_089169660.1	alkene reductase	-
ACG10_RS21660	73261..73479	-	219	Protein_75	hypothetical protein	-
ACG10_RS21665	73867..74559	+	693	Protein_76	ABC-F family ATP-binding cassette domain-containing protein	-
ACG10_RS21670	74823..75272	+	450	WP_089169991.1	DUF2384 domain-containing protein	Antitoxin
ACG10_RS21675	75269..75706	+	438	WP_089169992.1	RES family NAD+ phosphorylase	Toxin
ACG10_RS21680	75718..76113	+	396	WP_089169993.1	hypothetical protein	-
ACG10_RS21685	76527..77426	-	900	WP_089169994.1	LysR family transcriptional regulator	-
ACG10_RS21690	77535..78281	+	747	WP_089169995.1	SDR family oxidoreductase	-
ACG10_RS21695	78372..78629	-	258	WP_089169996.1	hypothetical protein	-
ACG10_RS21700	78928..79401	+	474	WP_089169997.1	hypothetical protein	-
ACG10_RS21705	79663..79935	-	273	WP_089169998.1	HU family DNA-binding protein	-
ACG10_RS21710	79999..80226	-	228	WP_089169999.1	hypothetical protein	-
ACG10_RS21715	80440..80694	+	255	WP_089170000.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..306103	306103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-140)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (97-143)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15878.22 Da
• Isoelectric Point: 5.4736

>T55088 WP_089169992.1 NZ_CP011836:75269-75706 [Azotobacter chroococcum]
VILWRISAYADLSGIGGLRASGRWHHLGRPVVYAADSPAGAMLEVLVHLEIDPEDFPTTLQLIRIELPDPVSRAELPPLP
EHWKSDPEVTRALGDHFLDGRSALLLPVPSAIIPCTTNYLFNPAHPEASGASIHVEKFPLDSRLL

Nucleotide

• Length: 438 bp

>T55088 NZ_CP011836:75269-75706 [Azotobacter chroococcum]
GTGATTCTGTGGCGGATCAGCGCCTACGCGGACCTCAGCGGAATCGGTGGCCTGCGCGCCAGTGGCCGGTGGCACCACCT
TGGCAGGCCGGTCGTTTACGCGGCGGACAGTCCTGCCGGTGCGATGCTGGAGGTACTGGTCCACCTGGAAATCGACCCCG
AGGACTTCCCCACAACGCTCCAGTTGATCCGCATCGAGCTTCCAGACCCGGTTTCGCGGGCCGAGCTTCCGCCGCTGCCG
GAGCACTGGAAGTCTGACCCGGAAGTCACGCGCGCCTTGGGAGACCACTTTCTGGATGGGCGGAGCGCTCTGCTGCTCCC
GGTGCCGAGCGCCATCATCCCTTGCACGACCAACTACCTGTTCAACCCGGCGCACCCCGAGGCCAGCGGGGCGAGCATTC
ACGTAGAAAAATTCCCCCTCGATTCCCGTCTCCTTTGA

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16966.54 Da
• Isoelectric Point: 6.7345

>AT55088 WP_089169991.1 NZ_CP011836:74823-75272 [Azotobacter chroococcum]
MLAEVLRDHGYTAYRARLQSLLDIPESATDFEIHTRITKGFPATRLVQLTEAGVVTPLERDQIIPLRTLKSRIERDQPLT
VEESDRFFRAAHITAMAEAVFGDREKAKRWLSKPKERFSGLTPMQMLTTQQGTSQVEEMLLQIVEGLAL

Nucleotide

• Length: 450 bp

>AT55088 NZ_CP011836:74823-75272 [Azotobacter chroococcum]
ATGCTTGCTGAAGTCCTGCGTGACCATGGCTACACCGCATACCGGGCACGTCTTCAAAGCCTGCTGGACATCCCGGAGTC
GGCAACCGACTTTGAAATCCACACCCGAATCACCAAGGGCTTTCCTGCGACCCGCCTGGTGCAACTGACCGAGGCCGGGG
TGGTCACGCCGCTCGAGCGCGATCAGATCATCCCCCTGAGAACCCTCAAGAGCCGTATCGAGCGCGATCAGCCGTTGACC
GTGGAGGAAAGCGACCGGTTTTTTCGCGCTGCCCATATCACGGCAATGGCCGAGGCTGTCTTCGGCGACCGCGAGAAGGC
CAAACGTTGGCTCTCCAAGCCCAAGGAGCGGTTTTCCGGCCTGACGCCCATGCAGATGCTCACCACCCAGCAGGGCACCA
GCCAGGTGGAGGAAATGCTGCTGCAGATCGTCGAAGGCCTGGCCCTGTGA