Detailed information of TA system TA055087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/Phd(antitoxin)
• Location: 25368..25966
• Replicon: plasmid pacX50fB3
• Accession: NZ_CP011836
• Organism: Azotobacter chroococcum strain B3

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: ACG10_RS21410
• Protein ID: WP_157731506.1
• Coordinates: 25625..25966 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0C4WSR8
• Locus tag: ACG10_RS21405
• Protein ID: WP_040107312.1
• Coordinates: 25368..25628 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACG10_RS21395	20453..22294	+	1842	WP_089169974.1	amidohydrolase	-
ACG10_RS21400	22794..24986	+	2193	WP_089169975.1	catalase/peroxidase HPI	-
ACG10_RS21405	25368..25628	+	261	WP_040107312.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ACG10_RS21410	25625..25966	+	342	WP_157731506.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACG10_RS21415	26053..26352	+	300	WP_040107314.1	hypothetical protein	-
ACG10_RS21420	26538..27293	-	756	WP_040107315.1	precorrin-4 C(11)-methyltransferase	-
ACG10_RS21425	27290..27703	-	414	WP_040107316.1	cobalamin biosynthesis protein	-
ACG10_RS21430	27723..28406	-	684	WP_040107317.1	CbtA family protein	-
ACG10_RS21435	28414..28596	-	183	WP_040107318.1	CbtB-domain containing protein	-
ACG10_RS21440	29290..30339	+	1050	WP_040107319.1	cobalamin biosynthesis protein CobW	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..306103	306103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-99)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13111.96 Da
• Isoelectric Point: 5.1133

>T55087 WP_157731506.1 NZ_CP011836:25625-25966 [Azotobacter chroococcum]
MSFDVQFATTAETSLFSQIAHLEPYHSYDGAYEKLSTLVVEVIRRLADHPLGYPISAQVSELGIAHYRELNLNGYRVFYE
CDEEKRLVVVLLILGQRQSIEQQLIHYCLMFDR

Nucleotide

• Length: 342 bp

>T55087 NZ_CP011836:25625-25966 [Azotobacter chroococcum]
ATGAGCTTCGACGTTCAGTTTGCTACTACTGCCGAAACCAGCCTGTTTTCCCAGATCGCCCACCTAGAGCCGTACCACAG
CTATGACGGGGCCTATGAAAAGCTCTCCACCTTGGTGGTAGAAGTTATACGCCGCTTGGCCGATCACCCGTTGGGCTACC
CCATCAGTGCACAAGTCAGCGAGCTTGGGATCGCCCATTACCGGGAGCTCAATCTCAACGGCTACCGCGTCTTCTATGAG
TGCGATGAAGAAAAGCGTCTCGTGGTGGTCCTGCTTATCCTGGGGCAGCGACAGAGCATCGAGCAGCAGCTGATCCACTA
CTGCCTGATGTTTGATCGCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9543.04 Da
• Isoelectric Point: 9.0005

>AT55087 WP_040107312.1 NZ_CP011836:25368-25628 [Azotobacter chroococcum]
MKIETISFLKKHAASLDLSEPMVITQNGLPAYVIESYAERKQRDETIALVKLLALGSRDKEQGKLVSGDTLLDRLAQRCQ
QPKGTS

Nucleotide

• Length: 261 bp

>AT55087 NZ_CP011836:25368-25628 [Azotobacter chroococcum]
ATGAAAATCGAGACGATCAGCTTCTTGAAGAAGCACGCCGCATCGTTGGATCTGAGCGAGCCCATGGTGATCACCCAGAA
TGGTCTGCCCGCCTATGTCATCGAGTCCTATGCCGAACGCAAGCAGCGTGATGAGACGATTGCGCTCGTCAAGCTCCTGG
CATTGGGTTCCCGCGACAAAGAGCAAGGCAAGCTGGTGTCGGGTGACACGCTCCTGGATCGGTTGGCACAACGATGCCAG
CAACCCAAAGGAACTTCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0C4WSR8