Detailed information of TA system TA055084

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1728939..1729606
• Replicon: chromosome
• Accession: NZ_CP011835
• Organism: Azotobacter chroococcum strain B3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: ACG10_RS08070
• Protein ID: WP_089167831.1
• Coordinates: 1729415..1729606 (-)
• Length: 64 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ACG10_RS08065
• Protein ID: WP_089167830.1
• Coordinates: 1728939..1729361 (-)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACG10_RS08030	1724133..1724327	+	195	WP_089167825.1	hypothetical protein	-
ACG10_RS08035	1724424..1725698	+	1275	WP_089169818.1	site-specific integrase	-
ACG10_RS08040	1725699..1726142	+	444	WP_089167826.1	VRR-NUC domain-containing protein	-
ACG10_RS08045	1726186..1726749	+	564	WP_089169819.1	hypothetical protein	-
ACG10_RS08050	1726802..1727053	-	252	WP_089167827.1	Arc family DNA-binding protein	-
ACG10_RS08055	1727142..1727306	+	165	WP_089167828.1	Arc family DNA-binding protein	-
ACG10_RS08060	1727308..1728345	+	1038	WP_089167829.1	phage antirepressor N-terminal domain-containing protein	-
ACG10_RS23610	1728725..1728883	+	159	WP_157731415.1	hypothetical protein	-
ACG10_RS08065	1728939..1729361	-	423	WP_089167830.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ACG10_RS08070	1729415..1729606	-	192	WP_089167831.1	type II toxin-antitoxin system HicA family toxin	Toxin
ACG10_RS08075	1729758..1730078	+	321	WP_089167832.1	phage holin, lambda family	-
ACG10_RS08080	1730078..1730386	+	309	WP_089167833.1	HNH endonuclease	-
ACG10_RS08085	1730489..1730929	+	441	WP_089167834.1	hypothetical protein	-
ACG10_RS08090	1730939..1732621	+	1683	WP_089167835.1	terminase large subunit	-
ACG10_RS24100	1732618..1732779	+	162	WP_089167836.1	hypothetical protein	-
ACG10_RS08100	1732772..1734061	+	1290	WP_089167837.1	phage portal protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1702601..1755478	52877

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-61)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-63)

Sequences

Toxin

Protein

• Length: 64 a.a.
• Molecular weight: 7146.16 Da
• Isoelectric Point: 10.6196

>T55084 WP_089167831.1 NZ_CP011835:c1729606-1729415 [Azotobacter chroococcum]
MKYSEFRRWLEAQGVEFTKSRGGSSHFKIKAPNGKQTIFPNHGAKEIGEGLRKDIIKQLGLND

Nucleotide

• Length: 192 bp

>T55084 NZ_CP011835:c1729606-1729415 [Azotobacter chroococcum]
ATGAAATACAGCGAGTTCCGGCGATGGCTGGAGGCCCAAGGGGTTGAGTTCACGAAGTCACGCGGCGGTAGCAGCCACTT
CAAGATCAAAGCCCCGAACGGCAAACAGACGATCTTCCCGAATCATGGAGCCAAGGAAATCGGCGAAGGACTCAGGAAGG
ACATCATCAAGCAACTGGGCCTCAACGACTGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15226.45 Da
• Isoelectric Point: 6.8433

>AT55084 WP_089167830.1 NZ_CP011835:c1729361-1728939 [Azotobacter chroococcum]
MYDYPITVHSEAGHYWSSCPDIPEAHSAGDTIGMLLANAFDGIRLALTIYVDQGRSIPLASPAKAGQYVVSLPAQTVAKV
HLWNTMREQGLRVADLARLLGVSHPVATRLVDFEHVSKIEQIERALHALGKRLSVSVEAA

Nucleotide

• Length: 423 bp

>AT55084 NZ_CP011835:c1729361-1728939 [Azotobacter chroococcum]
ATGTACGACTATCCAATCACCGTGCACAGCGAAGCCGGCCATTATTGGTCGTCTTGCCCAGACATTCCGGAAGCTCACAG
CGCAGGCGATACCATTGGGATGCTGCTGGCCAATGCCTTCGATGGAATCCGCCTCGCGCTGACCATCTACGTTGACCAGG
GAAGAAGCATTCCACTCGCCTCTCCTGCCAAGGCAGGCCAATACGTTGTTTCACTCCCGGCGCAGACGGTGGCCAAAGTG
CACCTATGGAACACGATGCGCGAACAGGGGCTGCGGGTGGCAGACTTGGCCAGATTGCTTGGAGTTTCTCACCCCGTAGC
CACCCGACTGGTCGACTTCGAACATGTATCGAAGATCGAGCAGATCGAACGAGCCCTGCATGCTCTCGGCAAGCGCCTGT
CTGTTTCCGTCGAGGCGGCCTGA