Detailed information of TA system TA055067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 362895..363508
• Replicon: plasmid pPF72-1
• Accession: NZ_CP011808
• Organism: Pandoraea faecigallinarum strain DSM 23572

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0H3X064
• Locus tag: AB870_RS24745
• Protein ID: WP_047909252.1
• Coordinates: 363209..363508 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AB870_RS24740
• Protein ID: WP_174554749.1
• Coordinates: 362895..363206 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB870_RS24710	357976..359700	+	1725	WP_064674964.1	hypothetical protein	-
AB870_RS24715	359812..360033	+	222	Protein_340	transposase	-
AB870_RS26090	360229..361041	-	813	Protein_341	IS5 family transposase	-
AB870_RS24730	361124..361705	-	582	Protein_342	IS6 family transposase	-
AB870_RS26095	361723..362040	+	318	Protein_343	transposase	-
AB870_RS24735	362232..362734	+	503	Protein_344	IS5 family transposase	-
AB870_RS26100	362737..362898	+	162	Protein_345	transposase	-
AB870_RS24740	362895..363206	-	312	WP_174554749.1	putative addiction module antidote protein	Antitoxin
AB870_RS24745	363209..363508	-	300	WP_047909252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AB870_RS26675	363632..363919	-	288	Protein_348	IS3 family transposase	-
AB870_RS26110	364081..364368	+	288	Protein_349	transposase	-
AB870_RS24755	364381..365538	-	1158	WP_047909254.1	IS30 family transposase	-
AB870_RS24760	365603..366490	+	888	Protein_351	IS3 family transposase	-
AB870_RS24765	366519..367469	+	951	Protein_352	IS30 family transposase	-
AB870_RS24770	367526..368362	+	837	WP_047909257.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..386625	386625
	-	inside	IScluster/Tn	-	-	339803..366490	26687

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (50-94)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.86 Da
• Isoelectric Point: 10.2199

>T55067 WP_047909252.1 NZ_CP011808:c363508-363209 [Pandoraea faecigallinarum]
MTYTVKQLGEFSDWLQGLKNGLTRQRLVKRLRKAQLGNLGDVAPVGEGVFEMREHFGPGWRLYYVQRGELVIVMLGGGDK
STQQADIRRAIALAKSLED

Nucleotide

• Length: 300 bp

>T55067 NZ_CP011808:c363508-363209 [Pandoraea faecigallinarum]
ATGACCTATACGGTGAAACAACTCGGCGAGTTCTCCGATTGGCTCCAAGGCCTGAAGAACGGCCTGACACGGCAGCGCCT
CGTTAAGCGGCTGCGCAAGGCCCAGCTTGGCAACTTGGGCGACGTGGCGCCTGTAGGCGAAGGCGTGTTCGAGATGCGCG
AACATTTCGGGCCAGGCTGGCGCTTGTACTACGTTCAGCGCGGCGAGCTGGTGATCGTGATGCTGGGAGGCGGTGACAAG
TCCACCCAGCAGGCCGACATTCGGCGAGCCATTGCGTTGGCGAAATCCCTGGAGGATTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11155.73 Da
• Isoelectric Point: 5.1693

>AT55067 WP_174554749.1 NZ_CP011808:c363206-362895 [Pandoraea faecigallinarum]
MTKRIKVSELSEFDAAEYLNSEEEVAAYLTTVLEENDPALLAAAIGDIARARGMSQVAKDSGIAREALYKALRPGSEPRF
ETISRVCAALGVRLVAQPMHPAH

Nucleotide

• Length: 312 bp

>AT55067 NZ_CP011808:c363206-362895 [Pandoraea faecigallinarum]
ATGACCAAGAGAATTAAGGTTTCCGAACTCTCCGAGTTCGATGCGGCCGAATATCTGAACAGCGAAGAGGAAGTGGCGGC
GTACCTGACAACCGTTCTTGAAGAGAACGATCCGGCATTGCTGGCCGCTGCTATCGGTGACATTGCCCGTGCACGAGGGA
TGTCGCAGGTGGCGAAGGATTCTGGCATCGCGCGCGAAGCGCTGTACAAAGCGCTTCGGCCCGGCAGCGAACCGCGCTTC
GAGACGATCAGCCGCGTTTGCGCTGCCTTGGGCGTGCGCCTGGTGGCACAGCCGATGCATCCAGCGCACTAA

Structures

Toxin

• AlphaFold DB: A0A0H3X064