Detailed information of TA system TA055064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 271498..272039
• Replicon: plasmid pPF72-1
• Accession: NZ_CP011808
• Organism: Pandoraea faecigallinarum strain DSM 23572

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AB870_RS24280
• Protein ID: WP_174554748.1
• Coordinates: 271498..271824 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A0H3WZZ3
• Locus tag: AB870_RS24285
• Protein ID: WP_047909393.1
• Coordinates: 271821..272039 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB870_RS24260	267309..268022	-	714	WP_053059762.1	autoinducer binding domain-containing protein	-
AB870_RS24265	268006..268305	-	300	WP_047909196.1	hypothetical protein	-
AB870_RS24270	268892..269563	-	672	WP_084664331.1	recombinase family protein	-
AB870_RS26005	269712..270826	-	1115	Protein_249	IS3 family transposase	-
AB870_RS26655	270906..271250	-	345	Protein_250	transposase	-
AB870_RS24280	271498..271824	-	327	WP_174554748.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AB870_RS24285	271821..272039	-	219	WP_047909393.1	antitoxin MazE family protein	Antitoxin
AB870_RS26015	272150..272416	+	267	Protein_253	site-specific integrase	-
AB870_RS26020	272426..272614	-	189	Protein_254	IS30 family transposase	-
AB870_RS24290	272769..273992	+	1224	WP_047909197.1	IS21 family transposase	-
AB870_RS24295	273989..274855	+	867	WP_047909198.1	IS21-like element helper ATPase IstB	-
AB870_RS24300	274927..276465	-	1539	WP_047909199.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..386625	386625

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11531.56 Da
• Isoelectric Point: 7.1661

>T55064 WP_174554748.1 NZ_CP011808:c271824-271498 [Pandoraea faecigallinarum]
MMRGDFVTIAMQGDFGKPRPALVIQANQFGEHTSVTVLPITSTLAAAPLLRVTIQPGAENGLQKPSQVMVDKAMTVMREK
VGPAFGHIDADALVEVERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T55064 NZ_CP011808:c271824-271498 [Pandoraea faecigallinarum]
ATGATGCGCGGCGACTTCGTGACCATCGCCATGCAAGGTGACTTTGGCAAGCCAAGGCCCGCGTTGGTTATCCAAGCCAA
CCAGTTTGGCGAGCACACGAGCGTTACGGTATTACCCATCACTAGCACGCTGGCGGCCGCGCCGCTGCTTCGTGTCACGA
TACAACCGGGTGCCGAAAACGGATTGCAGAAGCCTTCGCAAGTGATGGTAGACAAGGCGATGACGGTGATGCGCGAAAAG
GTCGGGCCCGCTTTCGGCCACATCGACGCGGATGCGCTGGTGGAGGTCGAGCGCTGTTTGGCGGTGTTTCTAGGCATCGC
CAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8342.45 Da
• Isoelectric Point: 7.1349

>AT55064 WP_047909393.1 NZ_CP011808:c272039-271821 [Pandoraea faecigallinarum]
MAHVNSRVRKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLVTQADTTDTAMQQFMDGALADVEGWTE

Nucleotide

• Length: 219 bp

>AT55064 NZ_CP011808:c272039-271821 [Pandoraea faecigallinarum]
ATGGCACACGTCAATTCACGAGTTCGGAAGCACCGCGATGCCCTTCGCATGGCAGGACTCCGCCCGGTTCAGATTTGGGT
GCCAGACACCCGTCGCCCAGATTTCGCGGAGGAATGCCGGCGCCAGTGCCGCCTAGTGACGCAGGCGGACACGACCGACA
CAGCCATGCAGCAGTTCATGGATGGAGCTTTGGCGGACGTGGAAGGCTGGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3WZZ3