Detailed information of TA system TA055061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 5337..6010
• Replicon: plasmid pPF72-1
• Accession: NZ_CP011808
• Organism: Pandoraea faecigallinarum strain DSM 23572

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AB870_RS23085
• Protein ID: WP_047909277.1
• Coordinates: 5606..6010 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0H3WYT0
• Locus tag: AB870_RS23080
• Protein ID: WP_047909276.1
• Coordinates: 5337..5609 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB870_RS23050	2048..2773	+	726	WP_047909271.1	IS6 family transposase	-
AB870_RS23055	2882..3280	-	399	WP_047909272.1	type II toxin-antitoxin system VapC family toxin	-
AB870_RS23060	3277..3522	-	246	WP_047909273.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
AB870_RS23065	3682..4313	-	632	Protein_4	site-specific integrase	-
AB870_RS23070	4534..4689	+	156	WP_157112497.1	hypothetical protein	-
AB870_RS23075	4805..5044	-	240	WP_047909275.1	hypothetical protein	-
AB870_RS23080	5337..5609	+	273	WP_047909276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AB870_RS23085	5606..6010	+	405	WP_047909277.1	type II toxin-antitoxin system VapC family toxin	Toxin
AB870_RS23090	6048..8294	-	2247	WP_053059768.1	tyrosine-type recombinase/integrase	-
AB870_RS23095	8402..9070	-	669	WP_047909278.1	hypothetical protein	-
AB870_RS23100	9071..9835	-	765	WP_047909279.1	hypothetical protein	-
AB870_RS26840	9844..10101	-	258	WP_167362753.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..386625	386625
	-	flank	IS/Tn	-	-	2138..2773	635

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (16-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14581.90 Da
• Isoelectric Point: 5.2389

>T55061 WP_047909277.1 NZ_CP011808:5606-6010 [Pandoraea faecigallinarum]
MSGYLLDKNIISDVIRSPDGAVARRIEQVGPKKIFTSIIVAAELRYGCAKKGSSKLLTKVEGILETLPVLPLDIPADAEY
GAIRTELEAAGQPIGMNDLLLAAQAYALGLTLVTDNTREFSRIRGLNVENWLER

Nucleotide

• Length: 405 bp

>T55061 NZ_CP011808:5606-6010 [Pandoraea faecigallinarum]
TTGAGCGGGTATCTGCTGGATAAGAACATCATCAGCGACGTGATCCGCAGCCCGGATGGGGCCGTTGCACGCCGCATTGA
GCAGGTCGGACCGAAGAAGATTTTCACCAGCATCATCGTCGCAGCCGAGTTGCGCTACGGCTGCGCAAAGAAGGGCTCTT
CCAAGCTGCTCACCAAAGTGGAGGGCATCCTTGAAACGCTCCCGGTGCTACCGCTGGATATTCCGGCTGACGCGGAATAC
GGTGCTATCCGCACCGAACTGGAAGCGGCTGGCCAACCGATCGGCATGAACGATCTGTTGCTCGCCGCACAAGCCTATGC
GCTCGGCCTCACACTAGTGACGGACAACACGCGCGAATTCAGCCGCATTCGTGGCCTCAACGTAGAGAATTGGCTGGAAA
GATAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10321.88 Da
• Isoelectric Point: 5.1881

>AT55061 WP_047909276.1 NZ_CP011808:5337-5609 [Pandoraea faecigallinarum]
MPTLRTPSRPKEAKLFRNNRSQAVRIPVEFELPGDRVLIHREGSKLIIEPVTRPTNIVELLAEWRKEAPLGPEDQFPAIE
DIPAQPEDIF

Nucleotide

• Length: 273 bp

>AT55061 NZ_CP011808:5337-5609 [Pandoraea faecigallinarum]
ATGCCTACCCTACGGACGCCTTCCCGACCCAAGGAGGCCAAGCTGTTTCGCAACAACCGCAGTCAGGCGGTGCGGATTCC
TGTGGAATTCGAGTTGCCAGGAGATCGTGTGCTAATCCACCGCGAAGGCAGCAAACTCATCATCGAGCCAGTGACTCGGC
CAACAAACATCGTCGAACTGCTGGCCGAGTGGAGGAAAGAAGCGCCGCTTGGGCCAGAAGATCAGTTCCCGGCCATTGAG
GACATTCCCGCCCAGCCGGAGGACATATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3WYT0