Detailed information of TA system TA055033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 99072..99597
• Replicon: chromosome
• Accession: NZ_CP011789
• Organism: Pseudomonas putida strain PC2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AB688_RS00475
• Protein ID: WP_063541519.1
• Coordinates: 99072..99368 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AB688_RS00480
• Protein ID: WP_063541521.1
• Coordinates: 99358..99597 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB688_RS00465	94805..95785	+	981	WP_063546592.1	integrase domain-containing protein	-
AB688_RS25980	95782..96057	+	276	WP_081255175.1	hypothetical protein	-
AB688_RS26680	96526..98082	-	1557	WP_146226828.1	hypothetical protein	-
AB688_RS00475	99072..99368	-	297	WP_063541519.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AB688_RS00480	99358..99597	-	240	WP_063541521.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AB688_RS00485	99754..100455	-	702	WP_063541524.1	arsenical resistance protein ArsH	-
AB688_RS00490	100465..100935	-	471	WP_063541526.1	arsenate reductase ArsC	-
AB688_RS00495	100963..102246	-	1284	WP_054891260.1	arsenic transporter	-
AB688_RS00500	102265..102612	-	348	WP_063541527.1	metalloregulator ArsR/SmtB family transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	tagR / ppkA / pppA / icmF1/tssM1 / dotU1 / hsiJ1 / hsiB1/vipA / hsiC1/vipB / hcp1 / hsiG1 / hsiH1 / clpV1 / vgrG1a	64257..267844	203587

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-81)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-64)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11499.36 Da
• Isoelectric Point: 10.1262

>T55033 WP_063541519.1 NZ_CP011789:c99368-99072 [Pseudomonas putida]
MTYSLEFDARALKEWRKLGDTVRQQLKKKLVAVLENPRVEANRLSDLPDCYKVKLRSSGYRLVYQVVEQQVVVFVVAVGK
REREEIYRAVAERVVSRE

Nucleotide

• Length: 297 bp

>T55033 NZ_CP011789:c99368-99072 [Pseudomonas putida]
ATGACCTATAGCCTCGAGTTTGATGCGCGAGCGCTGAAGGAGTGGCGCAAGCTGGGGGACACGGTTCGGCAGCAATTAAA
GAAAAAGTTGGTAGCGGTCTTGGAGAACCCTCGGGTTGAGGCCAATCGGTTGAGTGATCTTCCGGACTGCTACAAGGTCA
AGCTACGTAGCAGTGGGTATCGCTTGGTTTACCAGGTTGTGGAGCAGCAGGTTGTTGTGTTTGTAGTGGCTGTGGGTAAG
CGCGAGCGGGAGGAGATTTATCGAGCGGTTGCTGAGCGGGTAGTGAGCCGGGAGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8998.33 Da
• Isoelectric Point: 4.6310

>AT55033 WP_063541521.1 NZ_CP011789:c99597-99358 [Pseudomonas putida]
MASINVRIDDELKARAYRELEKLGVTPSELMRQALQYVAERGQLPFKTVLVTDEDEALLATVRERLADPQRVKVSLDDL

Nucleotide

• Length: 240 bp

>AT55033 NZ_CP011789:c99597-99358 [Pseudomonas putida]
ATGGCATCCATCAACGTCCGTATCGACGACGAACTCAAGGCGCGCGCCTACCGCGAATTGGAGAAGCTGGGAGTGACGCC
CTCCGAGCTGATGCGCCAAGCACTGCAATACGTTGCCGAGCGTGGGCAGCTTCCCTTCAAGACCGTGCTGGTCACAGATG
AGGACGAGGCACTGTTGGCAACTGTCAGGGAGCGACTGGCCGATCCGCAGCGTGTGAAGGTTTCACTGGATGACCTATAG