Detailed information of TA system TA055025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Phd(antitoxin)
• Location: 1618396..1618943
• Replicon: chromosome
• Accession: NZ_CP011775
• Organism: Vibrio vulnificus strain FORC_016

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2K7SRN5
• Locus tag: FORC16_RS07315
• Protein ID: WP_010448451.1
• Coordinates: 1618396..1618698 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U3A269
• Locus tag: FORC16_RS07320
• Protein ID: WP_005448240.1
• Coordinates: 1618686..1618943 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC16_RS22850	1614988..1615385	+	398	Protein_1396	transposase	-
FORC16_RS24140	1615386..1615445	-	60	Protein_1397	DUF3265 domain-containing protein	-
FORC16_RS07300	1615680..1616636	+	957	WP_060531432.1	IS110 family transposase	-
FORC16_RS07305	1616759..1617727	+	969	WP_081036190.1	IS30 family transposase	-
FORC16_RS07310	1617761..1618225	-	465	WP_060531669.1	ASCH domain-containing protein	-
FORC16_RS07315	1618396..1618698	-	303	WP_010448451.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FORC16_RS07320	1618686..1618943	-	258	WP_005448240.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FORC16_RS07325	1619122..1619364	+	243	WP_039553847.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FORC16_RS07330	1619372..1619671	+	300	WP_060531668.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FORC16_RS22860	1619733..1619825	-	93	WP_080772459.1	DUF3265 domain-containing protein	-
FORC16_RS07340	1620082..1621232	+	1151	WP_155521457.1	IS3 family transposase	-
FORC16_RS24155	1621262..1621429	+	168	Protein_1407	IS30 family transposase	-
FORC16_RS07350	1621927..1622598	-	672	WP_081036234.1	HAD-IA family hydrolase	-
FORC16_RS22865	1622595..1622744	-	150	WP_081036233.1	DUF3265 domain-containing protein	-
FORC16_RS07355	1623216..1623821	-	606	WP_060531664.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1610914..1786420	175506
	-	inside	IScluster/Tn	-	-	1615680..1626210	10530
	-	inside	Integron	-	-	1617761..1659080	41319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11625.52 Da
• Isoelectric Point: 5.1765

>T55025 WP_010448451.1 NZ_CP011775:c1618698-1618396 [Vibrio vulnificus]
MAEVIWTEPALSDLNDIAEYIALENIVAAKQLVQTIFSKVERLQTFPESGRIPPELEHLSYREVVVNPCRVFYKQDGDKV
FILFVMRAERDLRKFLLGKQ

Nucleotide

• Length: 303 bp

>T55025 NZ_CP011775:c1618698-1618396 [Vibrio vulnificus]
ATGGCTGAAGTAATCTGGACTGAACCAGCGTTATCTGACCTCAATGATATCGCTGAATACATCGCACTTGAAAATATCGT
AGCTGCAAAGCAGTTAGTACAAACGATCTTCTCCAAAGTCGAACGCCTACAAACTTTCCCAGAGTCAGGTCGTATTCCAC
CCGAGCTAGAACATTTAAGTTATCGTGAAGTTGTCGTTAATCCATGTCGTGTTTTCTATAAACAAGACGGTGACAAAGTG
TTTATTCTGTTTGTTATGCGTGCAGAGAGAGATTTGCGTAAGTTCTTGTTGGGTAAGCAATAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9606.10 Da
• Isoelectric Point: 6.7269

>AT55025 WP_005448240.1 NZ_CP011775:c1618943-1618686 [Vibrio vulnificus]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLVDVDDYEFMQNRLAILEGIARGERALADGKVVSHDEAKDKM
SKWLK

Nucleotide

• Length: 258 bp

>AT55025 NZ_CP011775:c1618943-1618686 [Vibrio vulnificus]
ATGAAAGTAGAACTAGTTACATCACTCAAACGTCAAGCAACTAAGATCCTCGCCGATCTCCACGACACCAAAGAACCAGT
GTTAATTACTGAGCATGGTAAGCCATCTGCGTATCTAGTTGATGTTGATGACTACGAATTTATGCAAAATCGTCTAGCTA
TTCTTGAGGGTATTGCACGAGGTGAGCGTGCACTAGCTGATGGTAAAGTTGTAAGTCATGATGAAGCCAAGGACAAAATG
TCAAAATGGCTGAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2K7SRN5

Antitoxin

• AlphaFold DB: A0A432DGX8