Detailed information of TA system TA055021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 171400..172078
• Replicon: chromosome
• Accession: NZ_CP011774
• Organism: Limnohabitans sp. 63ED37-2

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: L63ED372_RS00745
• Protein ID: WP_062401934.1
• Coordinates: 171400..171756 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A0P0LEV4
• Locus tag: L63ED372_RS00750
• Protein ID: WP_062401937.1
• Coordinates: 171758..172078 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L63ED372_RS00715	166447..166944	-	498	WP_062401925.1	transcription/translation regulatory transformer protein RfaH	-
L63ED372_RS00720	167072..167377	+	306	WP_062407456.1	hypothetical protein	-
L63ED372_RS00725	167814..168320	-	507	WP_062401928.1	type II toxin-antitoxin system YhaV family toxin	-
L63ED372_RS00730	168317..168646	-	330	WP_062407458.1	type II toxin-antitoxin system PrlF family antitoxin	-
L63ED372_RS16105	168727..168882	-	156	WP_082431746.1	type II toxin-antitoxin system RelE/ParE family toxin	-
L63ED372_RS00735	169273..170013	+	741	WP_197275294.1	YdcF family protein	-
L63ED372_RS00740	170032..171213	-	1182	WP_062407460.1	DNA-processing protein DprA	-
L63ED372_RS00745	171400..171756	+	357	WP_062401934.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L63ED372_RS00750	171758..172078	+	321	WP_062401937.1	helix-turn-helix domain-containing protein	Antitoxin
L63ED372_RS00755	172117..172839	+	723	WP_062401940.1	amidotransferase	-
L63ED372_RS00760	172901..173407	+	507	WP_062401943.1	peptide deformylase	-
L63ED372_RS00765	173414..174376	+	963	WP_062401945.1	methionyl-tRNA formyltransferase	-
L63ED372_RS00770	174376..175107	+	732	WP_062401947.1	AzlC family ABC transporter permease	-
L63ED372_RS00775	175104..175439	+	336	WP_062401950.1	AzlD domain-containing protein	-
L63ED372_RS00780	175548..176741	+	1194	WP_062401953.1	phosphoglycerate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-85)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13074.22 Da
• Isoelectric Point: 9.5457

>T55021 WP_062401934.1 NZ_CP011774:171400-171756 [Limnohabitans sp. 63ED37-2]
MSDLLKPLHWVGSSKKDLLAMPCDVVDVFGFALHLAQSGKKHDQTKPLKGFGGASVLEVVEDHMGDTFRAVYTVKIAERV
YVLHCFQKKSNKGIETPKQDMDLIRERLKAAQAHAKGA

Nucleotide

• Length: 357 bp

>T55021 NZ_CP011774:171400-171756 [Limnohabitans sp. 63ED37-2]
ATGTCTGATCTGCTGAAGCCACTGCACTGGGTGGGTTCCTCGAAAAAGGATCTCCTTGCCATGCCCTGTGACGTCGTCGA
CGTTTTCGGCTTTGCGTTGCATTTGGCGCAGTCAGGCAAGAAGCATGATCAGACTAAGCCTCTGAAGGGTTTTGGTGGAG
CCAGTGTTTTGGAGGTGGTTGAAGACCACATGGGCGATACCTTCCGTGCCGTCTATACGGTCAAGATCGCAGAGAGGGTC
TACGTTTTGCATTGTTTCCAAAAGAAGTCCAATAAAGGCATTGAGACACCAAAGCAAGACATGGATTTGATTCGAGAACG
TTTGAAGGCAGCGCAAGCGCATGCCAAAGGAGCCTGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11719.75 Da
• Isoelectric Point: 10.4692

>AT55021 WP_062401937.1 NZ_CP011774:171758-172078 [Limnohabitans sp. 63ED37-2]
MIEIEKGSTNVYEDLGLPDAAEMQVKATLAAKIGEIIKHRHLTQIQAAEILGMPQPKLSGMLRGQFRGISEAKMLECMNR
LGRDVQIVVRKPSRSRTTGRTSVVFA

Nucleotide

• Length: 321 bp

>AT55021 NZ_CP011774:171758-172078 [Limnohabitans sp. 63ED37-2]
ATGATCGAAATTGAAAAAGGATCTACCAATGTCTACGAAGACCTTGGTTTGCCAGATGCTGCCGAGATGCAGGTTAAGGC
GACCTTGGCCGCCAAGATCGGCGAAATCATTAAGCATCGTCATCTGACCCAAATTCAAGCTGCTGAGATCCTGGGCATGC
CGCAGCCCAAACTCTCTGGCATGTTGCGCGGTCAGTTTCGGGGTATCAGTGAGGCGAAGATGCTGGAGTGCATGAATCGG
CTAGGCCGGGATGTTCAAATTGTGGTGCGTAAGCCATCTCGTTCGCGGACAACAGGGCGGACAAGCGTGGTATTTGCATA
G

Structures

Antitoxin

• AlphaFold DB: A0A0P0LEV4