Detailed information of TA system TA055014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 9261..10711
• Replicon: plasmid p1
• Accession: NZ_CP011771
• Organism: Croceicoccus naphthovorans strain PQ-2

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: G6EKU5
• Locus tag: AB433_RS17655
• Protein ID: WP_007015873.1
• Coordinates: 9470..10711 (+)
• Length: 414 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: AB433_RS17650
• Protein ID: WP_007015874.1
• Coordinates: 9261..9473 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB433_RS17610	4284..4472	-	189	WP_150113442.1	hypothetical protein	-
AB433_RS17615	4786..5129	+	344	Protein_10	thermonuclease family protein	-
AB433_RS17620	5607..5942	+	336	WP_006949404.1	single-stranded DNA-binding protein	-
AB433_RS17625	6444..7001	+	558	WP_037486448.1	hypothetical protein	-
AB433_RS17630	7119..7607	+	489	WP_037486447.1	MucR family transcriptional regulator	-
AB433_RS17635	7673..8182	-	510	WP_037486446.1	GCN5 family acetyltransferase	-
AB433_RS17640	8186..8485	-	300	WP_037486444.1	DUF1778 domain-containing protein	-
AB433_RS17645	8561..8923	+	363	WP_037486442.1	hypothetical protein	-
AB433_RS17650	9261..9473	+	213	WP_007015874.1	helix-turn-helix transcriptional regulator	Antitoxin
AB433_RS17655	9470..10711	+	1242	WP_007015873.1	type II toxin-antitoxin system HipA family toxin	Toxin
AB433_RS17660	11093..11374	-	282	WP_007015872.1	hypothetical protein	-
AB433_RS17665	11938..12993	+	1056	WP_037486491.1	DUF2493 domain-containing protein	-
AB433_RS17670	13460..13771	+	312	WP_007015869.1	hypothetical protein	-
AB433_RS17675	13899..14126	-	228	WP_007015868.1	hypothetical protein	-
AB433_RS17680	14601..14891	+	291	WP_007015867.1	ribbon-helix-helix protein, CopG family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..194680	194680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (147-378)

Antitoxin

• Cro/C1-type helix-turn-helix domain (7-61)

Sequences

Toxin

Protein

• Length: 414 a.a.
• Molecular weight: 45341.27 Da
• Isoelectric Point: 6.4311

>T55014 WP_007015873.1 NZ_CP011771:9470-10711 [Croceicoccus naphthovorans]
MSSRLTVWWDGRVVGALYLDPFGETQFAYDPGWLTELRAPALSFSLPKQVEPFHRRECQPFFGGILPEESQRTAIARALG
VSADNEFRLLEHLGGEVAGALTLLPEGEAPTPHSAAAPKPLDDDRLIQLLDHLPMRPMLAGEDGLRLSLAGAQSKLPVLL
IDRQVALPAPGQPTSHILKPPITRFPWTTENEYFCMSLARAIGLDVAPVEMRTVGDRSFLLISRYDRTIGPSGELVRLHQ
EDFAQALGVPSHRKYASEGGPNFPDCFALLRRAATRPPRDILRLLDAAIFNLIIGNADAHAKNFSLLHKDGAIELAPLYD
LLSTTLYPELSPKLAMKIGGKHLLNELEPRHWDKFAVDCGLGAPFVRTRIRQLCEAVIAGLDNEFTGPTWAADGFVPVIA
LVRERAGLLGGKL

Nucleotide

• Length: 1242 bp

>T55014 NZ_CP011771:9470-10711 [Croceicoccus naphthovorans]
ATGAGCAGCAGGCTCACCGTCTGGTGGGACGGCCGTGTGGTCGGCGCCCTCTATCTCGACCCCTTCGGCGAAACCCAGTT
TGCCTATGATCCAGGCTGGCTGACAGAGCTTCGCGCTCCTGCGCTTTCATTCTCGCTACCCAAGCAGGTCGAGCCATTCC
ATCGTCGGGAATGCCAGCCTTTCTTTGGCGGCATTCTGCCCGAGGAAAGCCAGCGTACTGCCATCGCCCGCGCACTTGGT
GTCTCCGCCGACAATGAATTCCGGCTGCTCGAGCATCTCGGCGGTGAAGTGGCAGGCGCTTTGACGCTCCTTCCTGAAGG
CGAAGCCCCCACTCCCCATAGCGCCGCAGCGCCCAAACCTCTCGATGATGATCGTCTGATCCAGTTGCTCGATCATTTGC
CGATGCGTCCAATGCTGGCGGGCGAGGATGGGTTGCGGCTGTCATTGGCCGGGGCACAATCGAAACTTCCGGTGCTTCTG
ATCGACAGGCAGGTTGCCCTGCCAGCACCCGGGCAGCCGACCAGTCATATCCTCAAACCACCGATCACGCGTTTCCCCTG
GACGACCGAGAACGAATACTTCTGCATGTCGCTCGCCCGCGCCATCGGCCTTGACGTTGCACCGGTCGAAATGCGTACGG
TTGGCGATCGTTCATTTCTGCTGATCAGTCGCTATGATCGGACCATCGGTCCGTCAGGCGAACTGGTCCGCCTGCATCAG
GAAGACTTCGCGCAGGCGCTTGGCGTGCCGTCACACCGCAAATATGCGAGCGAAGGCGGGCCGAATTTTCCTGATTGCTT
CGCGCTGCTGCGCCGGGCAGCAACCCGTCCGCCACGCGACATCCTGCGGCTGCTCGATGCGGCAATCTTCAACCTGATCA
TCGGCAATGCTGATGCCCACGCCAAGAATTTTAGCCTCCTGCACAAGGACGGCGCGATCGAGCTTGCACCGCTCTACGAT
CTGCTTTCGACCACGCTTTACCCGGAACTCTCGCCGAAGCTCGCGATGAAGATTGGCGGGAAGCACCTCCTCAACGAACT
CGAGCCGCGTCATTGGGACAAGTTCGCTGTCGATTGCGGGCTTGGCGCCCCGTTCGTGCGGACCCGGATCAGGCAATTGT
GTGAGGCTGTCATTGCCGGCCTGGACAACGAATTTACTGGCCCTACTTGGGCGGCAGATGGATTCGTCCCTGTCATCGCG
CTTGTCCGTGAGCGCGCCGGGCTGCTCGGTGGGAAGTTGTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7442.71 Da
• Isoelectric Point: 10.3137

>AT55014 WP_007015874.1 NZ_CP011771:9261-9473 [Croceicoccus naphthovorans]
MDSNSLGLLVRRERKAQNLKQAELAAVSGVGVRFIVDLEAGKPTLQLGKVLQVITTLGCEVQIAPPASRS

Nucleotide

• Length: 213 bp

>AT55014 NZ_CP011771:9261-9473 [Croceicoccus naphthovorans]
ATGGACAGCAATTCACTTGGCCTCCTTGTGCGCCGCGAACGCAAGGCACAGAACCTGAAGCAGGCAGAGCTTGCGGCAGT
GAGCGGCGTTGGCGTGCGGTTTATTGTCGATCTCGAAGCTGGCAAGCCGACGCTGCAACTCGGCAAGGTGCTTCAGGTCA
TCACGACGCTCGGCTGCGAAGTCCAGATCGCCCCGCCTGCGAGCCGGTCATGA

Structures

Toxin

• AlphaFold DB: G6EKU5