Detailed information of TA system TA054845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1256917..1257834
• Replicon: chromosome
• Accession: NZ_CP011686
• Organism: Bacillus velezensis strain G341

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: ABH13_RS06515
• Protein ID: WP_032866111.1
• Coordinates: 1257088..1257834 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: ABH13_RS06510
• Protein ID: WP_003154807.1
• Coordinates: 1256917..1257087 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABH13_RS06470	1252051..1252563	+	513	WP_007407279.1	sigma-70 family RNA polymerase sigma factor	-
ABH13_RS19855	1252676..1252897	+	222	Protein_1215	terminase	-
ABH13_RS06475	1252901..1253773	+	873	Protein_1216	hypothetical protein	-
ABH13_RS06480	1253786..1254157	+	372	WP_007407260.1	hypothetical protein	-
ABH13_RS06485	1254162..1254359	+	198	WP_012117366.1	XkdX family protein	-
ABH13_RS06490	1254415..1255176	+	762	WP_047935637.1	hypothetical protein	-
ABH13_RS06495	1255228..1255491	+	264	WP_003154815.1	hemolysin XhlA family protein	-
ABH13_RS06500	1255505..1255768	+	264	WP_003154813.1	phage holin	-
ABH13_RS06505	1255782..1256660	+	879	WP_020955695.1	N-acetylmuramoyl-L-alanine amidase	-
ABH13_RS06510	1256917..1257087	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
ABH13_RS06515	1257088..1257834	-	747	WP_032866111.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
ABH13_RS06520	1257938..1258936	-	999	WP_020955696.1	inorganic phosphate transporter	-
ABH13_RS06525	1258949..1259566	-	618	WP_003154804.1	DUF47 domain-containing protein	-
ABH13_RS06530	1259851..1261167	-	1317	WP_007407254.1	amino acid permease	-
ABH13_RS06535	1261490..1262440	+	951	WP_047935639.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1242959..1266097	23138

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29095.60 Da
• Isoelectric Point: 4.7755

>T54845 WP_032866111.1 NZ_CP011686:c1257834-1257088 [Bacillus velezensis]
MLMFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T54845 NZ_CP011686:c1257834-1257088 [Bacillus velezensis]
ATGCTTATGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT54845 WP_003154807.1 NZ_CP011686:c1257087-1256917 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT54845 NZ_CP011686:c1257087-1256917 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14