Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 495773..496410 Replicon chromosome
Accession NZ_CP011686
Organism Bacillus velezensis strain G341

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ABH13_RS02535 Protein ID WP_003156187.1
Coordinates 496060..496410 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag ABH13_RS02530 Protein ID WP_003156188.1
Coordinates 495773..496054 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ABH13_RS02510 492138..492737 - 600 WP_020953896.1 rhomboid family intramembrane serine protease -
ABH13_RS02515 492830..493195 + 366 WP_007410229.1 holo-ACP synthase -
ABH13_RS02520 493360..494367 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
ABH13_RS02525 494484..495653 + 1170 WP_032866888.1 alanine racemase -
ABH13_RS02530 495773..496054 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ABH13_RS02535 496060..496410 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ABH13_RS02540 496528..497349 + 822 WP_003156182.1 STAS domain-containing protein -
ABH13_RS02545 497354..497719 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
ABH13_RS02550 497722..498123 + 402 WP_003156178.1 anti-sigma regulatory factor -
ABH13_RS02555 498135..499142 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
ABH13_RS02560 499206..499535 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
ABH13_RS02565 499532..500014 + 483 WP_003156173.1 anti-sigma B factor RsbW -
ABH13_RS02570 499980..500768 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
ABH13_RS02575 500768..501370 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T54844 WP_003156187.1 NZ_CP011686:496060-496410 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T54844 NZ_CP011686:496060-496410 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT54844 WP_003156188.1 NZ_CP011686:495773-496054 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT54844 NZ_CP011686:495773-496054 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References