Detailed information of TA system TA054804

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3617451..3618115
• Replicon: chromosome
• Accession: NZ_CP011657
• Organism: Citrobacter freundii strain CAV1741

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: AB183_RS19340
• Protein ID: WP_047715922.1
• Coordinates: 3617774..3618115 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: A0A1D3BNC1
• Locus tag: AB183_RS19335
• Protein ID: WP_032666156.1
• Coordinates: 3617451..3617753 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB183_RS19290	3612868..3613689	+	822	WP_032666166.1	DUF945 domain-containing protein	-
AB183_RS19295	3613906..3614607	+	702	WP_032666165.1	WYL domain-containing protein	-
AB183_RS19300	3614648..3614884	+	237	WP_001144029.1	hypothetical protein	-
AB183_RS19305	3614884..3615327	+	444	WP_032666164.1	hypothetical protein	-
AB183_RS19310	3615351..3615818	+	468	WP_032666163.1	hypothetical protein	-
AB183_RS19315	3615895..3616134	+	240	WP_032666162.1	DUF905 domain-containing protein	-
AB183_RS19320	3616237..3616695	+	459	WP_032666161.1	antirestriction protein	-
AB183_RS19325	3616711..3617187	+	477	WP_047715925.1	RadC family protein	-
AB183_RS19330	3617196..3617417	+	222	WP_047715924.1	DUF987 domain-containing protein	-
AB183_RS19335	3617451..3617753	+	303	WP_032666156.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AB183_RS19340	3617774..3618115	+	342	WP_047715922.1	toxin	Toxin
AB183_RS19350	3618478..3618984	+	507	WP_044700923.1	G/U mismatch-specific DNA glycosylase	-
AB183_RS19355	3619015..3621012	-	1998	WP_003846276.1	TonB-dependent receptor	-
AB183_RS19360	3621023..3622072	-	1050	WP_044700921.1	YncE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3601171..3618115	16944

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-99)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13019.14 Da
• Isoelectric Point: 10.2220

>T54804 WP_047715922.1 NZ_CP011657:3617774-3618115 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVVVWQMLLTRLLEQHYGLTLNDTPFGDERVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQVTGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T54804 NZ_CP011657:3617774-3618115 [Citrobacter freundii]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCTTGTCACCCGTAGTTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACACTGAACGATACGCCGTTCGGTGATGAAAGGGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCCCCTTACCTTCGGGCTGTCGATATTCTGCGAGCGCGGCAGGTAACTGGCTTGTTGCGGCAAAG
CCGTAAAAATTCGGTACGGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11186.65 Da
• Isoelectric Point: 6.3129

>AT54804 WP_032666156.1 NZ_CP011657:3617451-3617753 [Citrobacter freundii]
MGPAAEYTPRLGARLVQEGNRLHYLADRASITGMFSDAECRKLDDTFPHFIRQMESMLATGELSPQHAHCVTLYHNGFTC
EADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 303 bp

>AT54804 NZ_CP011657:3617451-3617753 [Citrobacter freundii]
CTGGGGCCTGCAGCGGAATACACGCCGCGCCTAGGAGCTCGTCTGGTGCAGGAGGGCAACCGGCTGCATTATCTGGCTGA
CCGGGCCAGCATCACCGGTATGTTCAGTGACGCCGAATGTCGGAAGCTGGATGACACATTCCCGCATTTTATCCGCCAGA
TGGAATCGATGCTGGCCACCGGTGAACTCAGCCCTCAACATGCCCACTGCGTCACCCTGTACCACAACGGTTTCACCTGC
GAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1D3BNC1