54797

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 1837026..1837705 Replicon chromosome
Accession NZ_CP011657
Organism Citrobacter freundii strain CAV1741

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag AB183_RS10850 Protein ID WP_003031349.1
Coordinates 1837364..1837705 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag AB183_RS10845 Protein ID WP_003031347.1
Coordinates 1837026..1837343 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB183_RS10825 1833119..1835116 - 1998 WP_016245729.1 choline BCCT transporter BetT -
AB183_RS28070 1835620..1835811 + 192 Protein_1747 DUF905 family protein -
AB183_RS10830 1835825..1836286 + 462 WP_003031344.1 antirestriction protein -
AB183_RS10835 1836302..1836778 + 477 WP_003031345.1 RadC family protein -
AB183_RS10840 1836787..1837008 + 222 WP_003031346.1 DUF987 domain-containing protein -
AB183_RS10845 1837026..1837343 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
AB183_RS10850 1837364..1837705 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
AB183_RS10860 1838335..1838799 - 465 WP_044702482.1 isoprenylcysteine carboxylmethyltransferase family protein -
AB183_RS10865 1838991..1840439 - 1449 WP_044702495.1 EAL domain-containing protein -
AB183_RS10870 1840781..1841254 - 474 WP_044702484.1 hypothetical protein -
AB183_RS10875 1841229..1841960 - 732 WP_044702485.1 winged helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1822653..1849601 26948


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T54797 WP_003031349.1 NZ_CP011657:1837364-1837705 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T54797 NZ_CP011657:1837364-1837705 [Citrobacter freundii]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATAGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGTAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT54797 WP_003031347.1 NZ_CP011657:1837026-1837343 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT54797 NZ_CP011657:1837026-1837343 [Citrobacter freundii]
ATGAGCAAAATCACATGGGGTCTGCAGCGTGATATCACGCCACGTCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATGACCGGCAAGTTCAGTGACATCGAATACCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACTGGTGAACTCAGCACCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References