Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 81122..81765 Replicon plasmid pCAV1741-101
Accession NZ_CP011654
Organism Citrobacter freundii strain CAV1741

Toxin (Protein)


Gene name vagD Uniprot ID A0A6C0NE46
Locus tag AB183_RS00575 Protein ID WP_008322233.1
Coordinates 81122..81538 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag AB183_RS00580 Protein ID WP_001261276.1
Coordinates 81535..81765 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB183_RS00560 77192..78211 + 1020 WP_004113181.1 ribose ABC transporter permease -
AB183_RS00565 78289..79659 + 1371 WP_008322237.1 hypothetical protein -
AB183_RS00570 79656..80576 + 921 WP_008322235.1 carbohydrate kinase -
AB183_RS27595 80759..80928 - 170 Protein_85 DUF596 domain-containing protein -
AB183_RS00575 81122..81538 - 417 WP_008322233.1 type II toxin-antitoxin system VapC family toxin Toxin
AB183_RS00580 81535..81765 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AB183_RS00585 82018..83040 - 1023 WP_008322227.1 helicase UvrD -
AB183_RS00590 83126..83830 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
AB183_RS00595 84157..84657 + 501 WP_001752509.1 non-heme ferritin-like protein -
AB183_RS27610 84776..86004 + 1229 WP_148116659.1 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..100873 100873


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15054.58 Da        Isoelectric Point: 9.2957

>T54787 WP_008322233.1 NZ_CP011654:c81538-81122 [Citrobacter freundii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAASAVLVTNNVREFARVPGLMLEDWVK

Download         Length: 417 bp

>T54787 NZ_CP011654:c81538-81122 [Citrobacter freundii]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTACGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCTGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCAGTGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGATGCTGG
AAGACTGGGTAAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT54787 WP_001261276.1 NZ_CP011654:c81765-81535 [Citrobacter freundii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT54787 NZ_CP011654:c81765-81535 [Citrobacter freundii]
ATGCGCACAGTTTCTATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCATTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE46

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References