54755

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1222531..1223156 Replicon chromosome
Accession NZ_CP011647
Organism Klebsiella pneumoniae strain CAV1596

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag AB187_RS07425 Protein ID WP_002882817.1
Coordinates 1222773..1223156 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag AB187_RS07420 Protein ID WP_004150355.1
Coordinates 1222531..1222773 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB187_RS07395 1218522..1219121 + 600 WP_004151865.1 glucose-1-phosphatase -
AB187_RS07400 1219115..1219975 + 861 WP_002882807.1 virulence factor BrkB family protein -
AB187_RS07405 1219972..1220409 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
AB187_RS07410 1220454..1221395 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
AB187_RS07415 1221409..1222326 - 918 WP_002882812.1 alpha/beta hydrolase -
AB187_RS07420 1222531..1222773 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
AB187_RS07425 1222773..1223156 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
AB187_RS07430 1223330..1224259 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
AB187_RS07435 1224256..1224891 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
AB187_RS07440 1224888..1225790 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T54755 WP_002882817.1 NZ_CP011647:1222773-1223156 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T54755 NZ_CP011647:1222773-1223156 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT54755 WP_004150355.1 NZ_CP011647:1222531-1222773 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT54755 NZ_CP011647:1222531-1222773 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References